Pegasus® GC-HRT

This application note highlights the advantages of using LECO’s new Pegasus® GC-HRT to analyze complex matrices such as polychlorinated biphenyls in fish oil. The resolving power and sensitivity of the high resolution time of flight mass spectrometer with Folded Flight Path™ (FFP™) technology are critical for targeting specific analysis of trace components in complex mixtures.

In this study, various light crude oil samples were analyzed for aromatic, polycyclic aromatic hydrocarbon and heterocyclic compounds. These compounds were characterized using a combination of elemental formula determinations with exact mass calculations and spectral library similarity searches. High performance TOFMS provided a comprehensive profile of samples.

Dioxin analysis in a regulated fashion requires conformity to the specifications of EPA Method 1613b. In this note, samples destined for analysis for dioxin content are analyzed in a comprehensive fashion using high resolution GC/MS. The objective is to uncover what other Persistent Organic Pollutants (POPs) are present.

This note describes the analysis of the barbiturate, pentobarbital, by Gas Chromatography–Time-of-Flight Mass Spectrometry (GC-TOFMS). Pentobarbital is a popular short-acting barbiturate derivative used primarily as a sedative.

Polychlorinated dibenzo-p-dioxins and furans are some of the most toxic materials known. These persistent organic pollutants (POPs) have been linked to cancer and adverse reproductive and endocrine effects. This application note shows that the performance of the LECO Pegasus® GC-HRT meets or exceeds the requirements for dioxin analysis per the US EPA 1613b Protocol.

This note describes the analysis of some common drugs of abuse by Gas Chromatography—Time-of-Flight Mass Spectrometry (GC-TOFMS). In addition to using standards, two real-life samples of blood and urine extracts were analyzed to demonstrate the power of the technique in handling samples containing small amounts of drugs in demanding matrices.

In this note GC High Resolution Time-of-Flight Mass Spectrometer is applied to the identification and differential analysis of metabolites extracted from diseased livers of mice exposed to alcohol with and without fatty diets. The aim is to study the role of alcohol and PCBs on mice fed with an unsaturated fat diet, it is therefore important to identify metabolites that have significant abundance changes in mice liver induced by alcohol.

Among the most commonly found drugs in horse doping are the non-steroidal anti-inflammatory drugs (NSAIDs): ibuprofen, diclofenac, vedaprofen, indomethacin, and eltenac; and the appetite-enhancer clanobutin which acts on the digestive system and improves the appetite. This note presents the analysis of the methylated drugs using GCTOFMS.

This application note shows the analysis of a commercially available synthetic cannabinoid by Gas Chromatography-High Resolution Time of Flight Mass Spectrometry (GC-HRT). The identification of these compounds by GC-MS can be challenging as most of them are not present in commercially available mass spectral search libraries. It was shown that the most abundant analyte detected in the samples was not present in the mass spectral search database (NIST 2008) which was used for spectral searching. Both electron ionization (EI) and chemical ionization (CI) experiments were then conducted to verify the molecular ion. The data was used to perform formula searches which ultimately lead to the identification of a known cannabinoid receptor agonist.

In this application note Gas Chromatography High Resolution Time-of-Flight Mass Spectrometry (GCHRT) was used for the analysis of PBDEs in sediment and fish biota samples. The 13 samples were fortified with labelled PBDE standards and dissolved in 12 nonane for analysis. Chromatographic separations were performed using an Agilent 7890 GC and 7693 auto sampler. Mass spectral data were collected using the Pegasus GC-HRT, yielding a mass resolving power of 25,000 (FWHM).

Watch this video to see the quick and easy set-up process of the Pippin Prep, a new electrophoresis platform for the preparation of size-fractionated DNA samples. The step-by-step guide takes you through from out of the box to sample ready. The kit includes an LCD computer monitor, documentation and a rinse cassette.

This video clearly demonstrates all the steps for automated DNA size selection and collection using the Pippin Prep. The video covers how to test the system, set up a protocol for different cut sizes and load the gels. This system is a new electrophoresis platform using pre-cast agarose gel cassettes.

This video demonstrates how the Pippin Prep selects and collects 100bp, 200bp, 300bp and 400bp DNA fragments over 80 minutes. Gel Cassettes use an external DNA size standard run in a dedicated sample lane to determine the timing of size-selected collections. Four lanes are used for samples, and one extraction can be collected per lane according to user input size ranges. Sample lanes are physically separate and tapered to shorten run times and improve resolution.