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Animation showing how the Pippin Prep Runs to Automatically Select and Collect Different Sized DNA

25 Mar 2011
Animation showing how the Pippin Prep Runs to Automatically Select and Collect Different Sized DNA

This video demonstrates how the Pippin Prep selects and collects 100bp, 200bp, 300bp and 400bp DNA fragments over 80 minutes. Gel Cassettes use an external DNA size standard run in a dedicated sample lane to determine the timing of size-selected collections. Four lanes are used for samples, and one extraction can be collected per lane according to user input size ranges. Sample lanes are physically separate and tapered to shorten run times and improve resolution.

Pippin Prep DNA Size Selection System

Sage Science

The Pippin Prep is a preparative electrophoresis platform for collection of size-selected DNA samples. The system automates DNA size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries. The system collects fragments (depending on gel type) between 90 bp- 8kb. A higher quality of sample can be extracted, more reproducibly and with no cross-contamination, when compared to manual gel purification methods.

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Pegasus® GC-HRT

LECO Corp.

While some systems on the market offer users a choice between acquisition speed or resolution, the Pegasus GC-HRT provides scientists with an unprecedented combination of speed, resolution, mass accuracy, isotopic abundance, and dynamic range. This instrumentation utilizes LECO's own Folded Flight Path™ (FFP™) technology to allow users to achieve resolutions of up to 50,000. LECO's exclusive ChromaTOF-HRT™ software with Automated Peak Find and True Signal Deconvolution® provides seamless data handling and compatibility with the NIST Library.

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Animation showing how the Pippin Prep Runs to Automatically Select and Collect Different Sized DNA