DASbox® Mini Bioreactor System
The DASbox is a unique mini bioreactor system suitable for microbial and cell culture as well as stem cell applications. It is designed as a 4-fold system with up to twenty-four parallel operating bioreactors. With working volumes of 60 – 250 mL the DASbox is the optimal tool for advanced process development and Design of Experiments (DoE) applications. All critical process parameters can be precisely controlled. Liquid-fre…
Receive your quote directly from the manufacturer.
The DASbox is a unique mini bioreactor system suitable for microbial and cell culture as well as stem cell applications.
It is designed as a 4-fold system with up to twenty-four parallel operating bioreactors. With working volumes of 60 – 250 mL the DASbox is the optimal tool for advanced process development and Design of Experiments (DoE) applications. All critical process parameters can be precisely controlled. Liquid-free temperature control and exhaust condensation satisfy users with easy handling.
In addition to using industry standard glass bioreactors the DASbox can be equipped with Eppendorf BioBLU® 0.3 vessels, all fully instrumented single-use mini bioreactors.
Eppendorf DASbox® Mini Bioreactor System Features:
- Parallel set-up of up to 24 bioreactors;
- Excellent scalability and reproducibility in both microbial and cell culture applications;
- Supports industry standard glass bioreactors (DASbox Mini Bioreactor) as well as the BioBLU 0.3 Single-Use Vessels;
- Small working volumes save on the amount of cell material, media and supplements required;
- Extremely compact system with a footprint of only 7.5 cm (3 in) benchspace per vessel;
- Individual temperature control with liquid-free heating and cooling (Peltier);
- Liquid-free Peltier exhaust condenser with easy handling by automatic slide in activation and slide out deactivation mode;
- LC display with key process parameters and integrated alarm function simplifies monitoring;
- Fully mass flow-controlled gas mixing with individual gas mixture from Air, O2, CO2 and N2, each directable either to headspace or sparger;
- Standard sensors for precise measurement and control of temperature, pH, DO, level and ORP (redox potential); optical pH sensors available;
- Precise miniature variable speed pumps, continuous flow rates down to 0.3 mL/h;
- Sealed magnetic overhead drives for single-use vessels and direct overhead drives for autoclavable vessels; up- or downflow selectable;
- Optional pull-out system for enhanced accessibility of bioreactors and control unit.
Applications:
- Process development in cell culture and microbiology;
- Design of Experiments (DoE) Media optimization Clone and cell line screening, strain characterization.
Transforming bioprocess data into actionable intelligence
Managing the weight of bioprocessing data can often overwhelm laboratories, but effective data management is both achievable and critical to successful biopharmaceutical development.
This application eBook highlights advanced instruments and digital tools that integrate seamlessly into laboratory infrastructure, enabling continuous monitoring and streamlined data management. With remote accessibility, these solutions empower scientists to maintain oversight and control of bioprocessing data beyond the physical lab environment.
Download to learn:
- How to conduct accurate and efficient real-time monitoring of Chinese hamster ovarian (CHO) cultivation using the BioNsight®
- Case studies using automated technologies for sampling CHO and E.coli cultures to understand growth conditions, and improve experimental throughput.
- The potential of Raman spectroscopy to accurately track critical process parameters
Optimizing cell and gene therapy workflows
In this guide, Eppendorf provides detailed information on optimizing cell and gene therapy workflows and options for scaling up cell cultures with advanced bioreactor systems. Cell and gene therapies are emerging fields of transformative medicine where diseases are treated by restoring or reconditioning cells or genes. Cells and genetically engineered cells demonstrate vastly different properties than traditional medicines and surgery. Akin to “living drugs”, they can heal and replace diseased organs and thus have the potential to provide curative therapies for various diseases.
Controlled, Large-Scale Manufacturing of hiPSC-Derived Cardiomyocytes
In this application note, Eppendorf discusses how functional human cardiomyocytes, derived from a diverse set of disease hiPSC lines, can be manufactured at a large scale using a controlled, stirred-tank bioreactor approach.
Growth of Methanotrophic Bacteria in Chemostat Mode in Eppendorf BioBLU f Single-Use Vessels
To investigate the methanol metabolism of Methylomicrobium alcaliphilum researchers at the San Diego State University analyzed bacterial growth and substrate utilization in chemostat mode. They used a parallel DASbox® Mini Bioreactor System equipped with BioBLU 0.3f Single-Use Vessels. Parallel experimentation using single-use equipment saved time and reduced possible sources of fluctuation between replicates.
Production of Human Induced Pluripotent Stem CellDerived Cortical Neurospheres in the DASbox Mini Bioreactor System
Stem cell-derived cortical neurospheres are of great interest in basic neurobiology research and drug research. Cultivation in controlled, stirred-tank bioreactors can improve the homogeneity of neural spheroid cultures and allows culture scale-up. We used a DASbox Mini Bioreactor System for the generation of human induced pluripotent stem cell (hiPSC) spheroids and subsequent neural differentiation. Results from three independent bioprocesses demonstrate the reproducibility of the neural differentiation protocol. In a single DASbox Mini Bioreactor, we obtained 2 x 108 cells, demonstrating the potential of stirred-tank bioreactors for the generation of high numbers of iPSCderived neurospheres.
Culture of 3D Cell Aggregates in Perfusion in a DASbox Mini Bioreactor System
Three-dimensional (3D) cell aggregates are of great interest for many applications, including disease modeling, drug toxicity assessment, and manufacturing of stem cell-based products. Stirred-tank bioreactors are promising culture systems for 3D cell aggregates, as they allow efficient establishment and maintenance of cell aggregates, process monitoring and control, and process scale-up to larger volumes. Furthermore, they can be operated in perfusion mode, which allows 3D cell aggregates to be sustained longer than in traditional batch cultures.
How to Successfully Culture 3D Cell Aggregates Using a DASbox Mini Bioreactor System
In this application note, learn how the DASbox Mini Bioreactor System can be used for bioprocess development and the intensification of 3D cell aggregates.
Parallel CHO Cell Cultivation in Eppendorf BioBLU Single-Use Bioreactors
The development of industrial bioprocesses is time-consuming and costly, yet the employment of single-use equipment alongside parallel experimentation considerably reduces development time. Parallel processing enables the testing of multiple parameters in one run, and single-use bioreactors omit the need to carry out extensive sterilization procedures. In this application note, Eppendorf, in collaboration with Dieter Eibel and his team at Zurich university, evaluate the DASbox Mini Bioreactor parallel cell culture system with BioBLU single-use vessels for their suitability in bioprocess development.
Development of a Scale-Down Model for rAAV Viral Vector Production Using a Sf9/BEV System
This application notes describes a study in which researchers developed a scale-down model for rAAV viral vector production in Sf9 cells using an Eppendorf DASbox® Mini Bioreactor System. Parallel experimentation in small working volumes allowed time- and cost-efficient evaluation of process performance.
Controlled Cultivation of Stem Cells – Factors to Consider When Thinking of Scale-Up
This white paper describes the process and difficulties that can be encountered during the scale-up of stem cells for therapy or research. It also describes how these problems can be resolved and offers case studies to support this.
Stem cell derived exome production in stirred-tank bioreactors
In this video, Eppendorf introduces exosomes as a promising cell-free therapy tool and describes the challenges behind the exosome production process.
Stem cells in modern medicine: From research to clinical applications
Watch this video from Eppendorf, titled: Stem cells in modern medicine: From research to clinical applications. This video was part of its panel discussion series titled 'Stimulating Growth, Cultivating Solutions'.
Eppendorf DASbox® Mini Bioreactor System
Watch this video to learn about the Eppendorf DASbox Mini Bioreactor System. The DASbox is a unique mini bioreactor system suitable for microbial and cell culture as well as stem cell applications. Its innovative design in blocks of four makes it an ideal tool for parallel processing and Design of Experiments.
Harnessing the latest bioprocessing technology in cell and gene therapy
Discover how the latest innovations in upstream bioprocessing are serving the cell and gene therapy industry
Stem cell research – Expert insights into the new frontier of modern medicine
Keep up to date with the latest techniques and technologies and techniques driving stem cell research into 2023
DASGIP® Control is now DASware® control 5
Parallel Processing Advances Bioprocess Development
