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Development of a Scale-Down Model for rAAV Viral Vector Production Using a Sf9/BEV System

Eppendorf
8 Apr 2017

This application notes describes a study in which researchers developed a scale-down model for rAAV viral vector production in Sf9 cells using an Eppendorf DASbox® Mini Bioreactor System. Parallel experimentation in small working volumes allowed time- and cost-efficient evaluation of process performance.

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DASbox® Mini Bioreactor System

Eppendorf

The DASbox is a unique mini bioreactor system suitable for microbial and cell culture as well as stem cell applications. It is designed as a 4-fold system with up to twenty-four parallel operating bioreactors. With working volumes of 60 – 250 mL the DASbox is the optimal tool for advanced process development and Design of Experiments (DoE) applications. All critical process parameters can be precisely controlled. Liquid-free temperature control and exhaust condensation satisfy users with easy handling. In addition to using industry standard glass bioreactors the DASbox can be equipped with Eppendorf BioBLU® 0.3 vessels, all fully instrumented single-use mini bioreactors. Eppendorf DASbox® Mini Bioreactor System Features: Parallel set-up of up to 24 bioreactors; Excellent scalability and reproducibility in both microbial and cell culture applications; Supports industry standard glass bioreactors (DASbox Mini Bioreactor) as well as the BioBLU 0.3 Single-Use Vessels; Small working volumes save on the amount of cell material, media and supplements required; Extremely compact system with a footprint of only 7.5 cm (3 in) benchspace per vessel; Individual temperature control with liquid-free heating and cooling (Peltier); Liquid-free Peltier exhaust condenser with easy handling by automatic slide in activation and slide out deactivation mode; LC display with key process parameters and integrated alarm function simplifies monitoring; Fully mass flow-controlled gas mixing with individual gas mixture from Air, O2, CO2 and N2, each directable either to headspace or sparger; Standard sensors for precise measurement and control of temperature, pH, DO, level and ORP (redox potential); optical pH sensors available; Precise miniature variable speed pumps, continuous flow rates down to 0.3 mL/h; Sealed magnetic overhead drives for single-use vessels and direct overhead drives for autoclavable vessels; up- or downflow selectable; Optional pull-out system for enhanced accessibility of bioreactors and control unit. Applications: Process development in cell culture and microbiology; Design of Experiments (DoE) Media optimization Clone and cell line screening, strain characterization.

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Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. 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Development of a Scale-Down Model for rAAV Viral Vector Production Using a Sf9/BEV System