Growth of Methanotrophic Bacteria in Chemostat Mode in Eppendorf BioBLU f Single-Use Vessels

As the smallest vessel of the Eppendorf single-use bioreactor family the BioBLU 0.3 is ideally suited for use as a scale-down model. Designed for use with the DASbox Mini Bioreactor System it is able to accelerate process development in biopharmaceutical and biotechnological applications using cell cultures and microorganisms.All critical process parameters can be precisely controlled and users benefit from easy handling, minimized set-up time and the elimination of cleaning and sterilization steps. The unique liquid-free exhaust gas condenser reduces negative effects caused by change of medium osmolarity and efficiently prevents blocking of the exhaust gas filter.BioBLU® 0.3 Single-Use Vessels Features: Fully instrumented single-use mini bioreactors for accelerated process development in cell culture and microbial applications. Excellent scalability and reproducibility. Small working volumes starting at 65 mL save on cell material, media and supplements. Used with the DASbox mini bioreactor system for parallel set-up of 4, 8, 12 or more bioreactors. Liquid-free exhaust condenser with easy handling by automatic click in activation and click out deactivation mode. Magnet-coupled overhead drive reduces the risk of contamination, up- or downflow. Headplate with 2x PG 13.5 ports, 4x integrated long dip tubes, 2x integrated short dip tubes, one thermowell, DO sensor port with permeable membrane. Operating with industry standard probes for precise measurement and control of temperature, pH, DO. Vessels are delivered pre-sterilized by ß-irradiation to dose exceeding 15 kGy (DIN EN ISO 11137-2:2007). All wetted materials are USP Class VI certified. Applications: Process development in cell culture and microbiology Design of Experiments (DoE) Media optimization Clone & cell line screening, strain characterization

Eppendorf BioBLU Single-use Vessels combine all the advantages of single-use technology with the trusted performance and scalability of a stirred-tank design.

The DASbox is a unique mini bioreactor system suitable for microbial and cell culture as well as stem cell applications. It is designed as a 4-fold system with up to twenty-four parallel operating bioreactors. With working volumes of 60 – 250 mL the DASbox is the optimal tool for advanced process development and Design of Experiments (DoE) applications. All critical process parameters can be precisely controlled. Liquid-free temperature control and exhaust condensation satisfy users with easy handling. In addition to using industry standard glass bioreactors the DASbox can be equipped with Eppendorf BioBLU® 0.3 vessels, all fully instrumented single-use mini bioreactors. Eppendorf DASbox® Mini Bioreactor System Features: Parallel set-up of up to 24 bioreactors; Excellent scalability and reproducibility in both microbial and cell culture applications; Supports industry standard glass bioreactors (DASbox Mini Bioreactor) as well as the BioBLU 0.3 Single-Use Vessels; Small working volumes save on the amount of cell material, media and supplements required; Extremely compact system with a footprint of only 7.5 cm (3 in) benchspace per vessel; Individual temperature control with liquid-free heating and cooling (Peltier); Liquid-free Peltier exhaust condenser with easy handling by automatic slide in activation and slide out deactivation mode; LC display with key process parameters and integrated alarm function simplifies monitoring; Fully mass flow-controlled gas mixing with individual gas mixture from Air, O2, CO2 and N2, each directable either to headspace or sparger; Standard sensors for precise measurement and control of temperature, pH, DO, level and ORP (redox potential); optical pH sensors available; Precise miniature variable speed pumps, continuous flow rates down to 0.3 mL/h; Sealed magnetic overhead drives for single-use vessels and direct overhead drives for autoclavable vessels; up- or downflow selectable; Optional pull-out system for enhanced accessibility of bioreactors and control unit. Applications: Process development in cell culture and microbiology; Design of Experiments (DoE) Media optimization Clone and cell line screening, strain characterization.