Enzymes Products & Reviews

The engineered PfuUltra II Fusion HS DNA Polymerase provides the worlds most accurate copies and the fastest extension times possible. PfuUltra II Fusion HS DNA Polymerase is PfuUltra hotstart DNA polymerase coupled with a fusion polymerase technology and ArchaeMaxx PCR enhancing factor.  The industry standard for ultra high fidelity PCR providing the most accurate copies of DNA  Dramatically reduced extension times allow f…

SP6 RNA Polymerase is a DNA-dependent RNA polymerase with high sequence specificity for SP6 promoter sequences. SP6 RNA polymerase synthesizes RNA 5' to 3' and incorporates 35S, 32P and 33P ribonucleotides. This enzyme is isolated from an overproducing recombinant E. coli clone.

SureStart Taq DNA polymerase is a hot start Taq DNA polymerase. SureStart Taq can be incorporated into PCR protocols previously optimized with Taq DNA polymerase, with little or no modification of cycling parameters or reaction conditions.

The PCR grade deoxynucleotide mix includes 25 mM of each standard dNTP (GATC) for use with any PCR application, including real-time quantitative PCR. These reactions buffers contain the optimal magnesium concentration for efficient PCR.

These reactions buffers contain the optimal magnesium concentration for efficient PCR.  Ideal for optimal PCR performance  Standard 10x formulation  Optimized for use with specific PCR enzymes

Discover AccuScript high-fidelity reverse transcriptase (RT). AccuScript delivers 3.7X more accuracy than other commercially available RTs and 6.6X more accuracy than AMV RT. The AccuScript PfuUltra II RT-PCR kit provides a complete system in applications that demand high fidelity, specificity, and throughput.

The Agilent AffinityScript Multiple Temperature Reverse Transcriptase (RT) system is optimized to produce high cDNA yields over the broadest temperature range. During analysis the multi-temp RT device also ensures high affinity to primer-template complexes. cDNA can be synthesized in amounts up to 20 kb.

The high-purity DpnI restriction enzyme is ideal for cloning and Southern blot analysis, and used for site-directed mutagenesis. Stringent quality control is achieved via pBluescript phagemid cloning assay. This assay tests the overall quality of the restriction enzyme used when cloning into the multiple cloning site of the pBluescript II phagemid vector.

Easy-A Hi-Fi Cloning Enzyme & Master Mix combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.

Combines the cloning efficiency of     Taq    DNA polymerase with the accuracy of Pfu DNA polymerase  Proofreading DNA polymerase formulation efficiently adds 3€™-A overhangs to PCR amplicons as efficiently as Taq  Easy-A-amplified PCR products are ready to clone into the TOPO TA Cloning vector and other T/U vectors  Fidelity equivalent to     Pfu    DNA polymerase and 6x more accurate than     Taq    DNA polymerase…

Combines the cloning efficiency of     Taq    DNA polymerase with the accuracy of Pfu DNA polymerase  Proofreading DNA polymerase formulation efficiently adds 3€™-A overhangs to PCR amplicons as efficiently as Taq  Easy-A-amplified PCR products are ready to clone into the TOPO TA Cloning vector and other T/U vectors  Fidelity equivalent to     Pfu    DNA polymerase and 6x more accurate than     Taq    DNA polymerase…

The PicoMaxx high fidelity PCR system reliably produces high PCR product yields on a wide variety of templates up to 10 kb. It also provides superior sensitivity by successfully amplifying samples where starting material is limited.  High sensitivity provides maximum PCR reliability  Consistent, robust amplification up to 10 kb  Superior efficiency and yield with our patented ArchaeMaxx polymerase-enhancing factor  Opti…

Widely used in DNA purification, Proteinase K is a broad-spectrum protease from Tritirachium album that is ideal for preparing chromosomal DNA. It cleaves peptide bonds where carbonyl groups are surrounded by aromatic, hydrophobic, or aliphatic amino acids.

Isopropyl-β-D-thiogalactoside (IPTG) is used in combination with X-gal to detect β-galactosidase activity in blue/white selection assays. When the appropriate vectors are used, blue white screening is an important tool for screening and selecting relevant colonies.

The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures.  Robust, cost-effective alternative to Taq  Equal to better yield with faster cycling conditions  Amplify up to 6 kb genomic DNA targets  Easily substituted…

Herculase II Fusion DNA Polymerases Pfu Ultra DNA Polymerases & PCR Master Mixes PfuUltra II Hotstart DNA Polymerases & PCR Master MixesHerculase II Fusion DNA PolymerasesThe Herculase II fusion DNA polymerase produces superior yields with extension times as short as 15 sec/kb (see Figure), even with genomic DNA targets up to 12kb. Herculase II fusion DNA polymerase helps overcome PCR challenges with successful amplification o…

Agilent Endoglycosidases (formerly ProZyme) release oligosaccharides from glycoproteins and some other substrates.

Agilent Exoglycosidases (formerly ProZyme) are glycoside hydrolase enzymes that cleave the glycosidic bonds at the terminal residues.

Agilent Glycosyltransferase (formerly ProZyme) kits are enzymes which are capable of adding terminal monosaccharides to released glycans or intact glycoproteins.

The GoTaq® G2 Hot Start Polymerase and Master Mixes simplify your reaction preparation, bringing you the convenience of room-temperature setup and the specificity of a hot start polymerase.   Ready-to-use Master Mix simplifies reaction setup and allows direct-to-gel loading. The optimized formulation provides amplification of a variety of targets, while eliminating non-specific products, resulting in higher yields and gr…

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues.   The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in addition…

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes).   Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification pri…

To shorten the time required to prepare samples for LC-MS/MS analysis, we have developed an optimized protocol in which rapid and efficient trypsin digestion is achieved at temperatures as high as 70°C. Using this approach, proteolytis reaction times are dramatically shortened, no chemical denaturants are added and off-line sample cleanup is unnecessary, leading to shorter preparation times and diminished sample losses.

IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It is an engineered recombinant protease overexpressed in E. coli that cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments. The protocol for a standard reaction is to add the IdeS Protease to the IgG sample, add 1 unit of IdeS Protease per 1µg of IgG to be digested…

IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus. It is an engineered recombinant protease overexpressed in E. coli. Like IdeS Protease, IdeZ Protease specifically cleaves IgG molecules below the hinge region to yield F(ab′)2 and Fc fragments. However, IdeZ Protease has significantly improved activity against mouse IgG2a and IgG3 subclasses compared to IdeS Protease.