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Sequencing Grade Modified Trypsin

Promega Corp.V5111, V5117, V5113Available: Worldwide

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in addition…

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Description

Description

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues.

The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in additional peptide fragments that could interfere with database analysis of the mass of fragments detected by mass spectrometry.

Sequencing Grade Trypsin has been manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion.

Sequencing Grade Modified Trypsin Features & Benefits:

TPCK Treatment Followed by Affinity Purification - Elimination of chymotrypsin activity enables distinct and consistent data.
Stability - Ensured up to five freeze-thaw cycles.
Reliable and Customer-Proven - Referenced in thousands of papers.
Alternative Formats - Flexibility depending on experimental design and scope.

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Sequencing Grade Modified Trypsin - Promega Corp. - Life Sciences

Sequencing Grade Modified Trypsin

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