Products & ReviewLife Sciences

LigaFast™ Rapid DNA Ligation System

Promega Corp.M8221, M8225

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification pri…

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Description

Description

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes).

Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg2+ addition prior to use.

LigaFast™ Rapid DNA Ligation System Features & Benefits:

Flexible - Use with 5´, 3´ or blunt-ended DNA inserts.
Fast - Ligation of cohesive ends in 5 minutes, blunt ends in 15 minutes at room temperature.
Convenient - No requirement to purify ligated DNA prior to heat-shock transformation in E. coli. Ligations conducted at room temperature.
Ready-To-Use - No additional buffer modifications required prior to use.
Efficient - Ligations performed using the LigaFast™ System are comparable to standard overnight ligations.
Blue/White Cloning Qualified - Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.

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LigaFast™ Rapid DNA Ligation System - Promega Corp. - Life Sciences

LigaFast™ Rapid DNA Ligation System

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