Enzymes Products & Reviews

Bioluminescent Assays to Detect NAD(P)+ and NAD(P)H Levels in Cells Homogeneous, single-reagent-addition assays Easily adapted for high throughput screening formats

In H2O. A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with tissue and cell extracts, including extracts containing detergents. Each vial contains 200 mM Imidazole, 100 mM Sodium Fluoride, 115 mM Sodium Molybdate, 100 mM Sodium Orthovanadate, and 400 mM Sodium Tartrate Dihydrate. Dilute 1:100 just prior to use. Pro…

OneTaq DNA Polymerase is an optimized blend of Taq and Deep VentR™ DNA polymerases for use with routine and difficult PCR experiments. The 3´→ 5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields with minimal optimization, regardless of a template’s GC content.OneTaq DNA…

Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate > 100-fold lower than that of Taq DNA Polymerase and 12-fold lower than that of Pyrococcus furiosus (Pfu) DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficul…

100 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water

Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types 1, 2, 3, and 4 or chromatographically purified collagenase (the latter usually combined with secondary enzymes such as elastase, hyaluronidase, etc). For best results, the precise mixture of proteolytic activities must be tailored to the tissue to…

PCRBIO Taq DNA Polymerase is an affordable, versatile and robust enzyme for all your everyday PCR applications including genotyping, screening and library construction. An enhanced 12-step purification strategy together with an optimised buffer system enable PCRBIO Taq DNA Polymerase to amplify with the highest speed, yield and specificity.

PCRBIO Taq Mix Red is an affordable, versatile and robust 2x mix for all your everyday PCR applications including genotyping, screening and library construction. The mix contains a red dye allowing reactions to be directly loaded onto agarose gels.

PCRBIO Classic Taq is a highly purified recombinant Taq DNA Polymerase for all your everyday PCR applications including genotyping, screening and library construction. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA.

BbsI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10119525.  NEB is excited to announce that all reaction buffers are now BSA-free. NEB began switching their BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). This polymerase has exceptional strand displacement and processive synthesis properties. The polymerase has an inherent 3´→5´ proofreading exonuclease activity. Source: An E. coli strain that carries the phi29 DNA Polymerase gene from bacteriophage phi29 Applications: •Replication requiring a high degree of strand displacement and/…

PCRBIO HS Taq Mix Red is an advanced antibody-mediated hot start DNA polymerase mix designed for fast, highly specific and sensitive PCR. The mix contains a red dye allowing reactions to be directly loaded onto agarose gels without additional loading buffer. Whether you need a hot start assay for high-throughput or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading 2x mix.

Eliminate the need for time-consuming DNA extraction methods with this simple, integrated extraction and amplification kit powered by the latest advances in hot start polymerase technology. PCRBIO Rapid Extract PCR Kit combines rapid DNA extraction with fast, highly specific DNA amplification in a convenient, easy-to-use format. Generate PCR-ready DNA in 15 minutes, without spin columns or hazardous chemicals.

Recombinant, high-purity endonucleases for genome editing experiments

HighTherm™ Reverse Transcriptase

Restriction Enzymes Dpn I is an ideal enzyme for cloning and Southern blot analysis High purity enzyme preparation Includes 10X universal reaction buffer Glogos II Autorad Markers Luminescent peel-off stickers used for accurately marking and aligning autoradiographs. Easily affixed to plastic wrap or paper, these convenient markers emit low-level luminescence rather than radioactivity, making the Glogos II m…

The SignalScout collection of phosphatase reagents includes the largest collection of human phosphatase expression clones and substrate trapping mutant expression clones.  Largest collection of active phosphatase enzymes, human phosphatase expression clones, and substrate trapping mutant expression clones  Purified GST-tagged active phosphatases for in vitro assay studies  Protein tyrosine, lipid, and dual specificity pho…

The SignalScout collection of phosphatase reagents includes the largest collection of human phosphatase expression clones and substrate trapping mutant expression clones.  Largest collection of active phosphatase enzymes, human phosphatase expression clones, and substrate trapping mutant expression clones  Purified GST-tagged active phosphatases for in vitro assay studies  Protein tyrosine, lipid, and dual specificity pho…

The SignalScout collection of phosphatase reagents includes the largest collection of human phosphatase expression clones and substrate trapping mutant expression clones.  Largest collection of active phosphatase enzymes, human phosphatase expression clones, and substrate trapping mutant expression clones  Purified GST-tagged active phosphatases for in vitro assay studies  Protein tyrosine, lipid, and dual specificity pho…

The SignalScout collection of phosphatase reagents includes the largest collection of human phosphatase expression clones and substrate trapping mutant expression clones.  Largest collection of active phosphatase enzymes, human phosphatase expression clones, and substrate trapping mutant expression clones  Purified GST-tagged active phosphatases for in vitro assay studies  Protein tyrosine, lipid, and dual specificity pho…

The SignalScout collection of phosphatase reagents includes the largest collection of human phosphatase expression clones and substrate trapping mutant expression clones.  Largest collection of active phosphatase enzymes, human phosphatase expression clones, and substrate trapping mutant expression clones  Purified GST-tagged active phosphatases for in vitro assay studies  Protein tyrosine, lipid, and dual specificity pho…

The SignalScout collection of phosphatase reagents includes the largest collection of human phosphatase expression clones and substrate trapping mutant expression clones.  Largest collection of active phosphatase enzymes, human phosphatase expression clones, and substrate trapping mutant expression clones  Purified GST-tagged active phosphatases for in vitro assay studies  Protein tyrosine, lipid, and dual specificity pho…

The SignalScout collection of phosphatase reagents includes the largest collection of human phosphatase expression clones and substrate trapping mutant expression clones.  Largest collection of active phosphatase enzymes, human phosphatase expression clones, and substrate trapping mutant expression clones  Purified GST-tagged active phosphatases for in vitro assay studies  Protein tyrosine, lipid, and dual specificity pho…

The SignalScout collection of phosphatase reagents includes the largest collection of human phosphatase expression clones and substrate trapping mutant expression clones.  Largest collection of active phosphatase enzymes, human phosphatase expression clones, and substrate trapping mutant expression clones  Purified GST-tagged active phosphatases for in vitro assay studies  Protein tyrosine, lipid, and dual specificity pho…

The SignalScout collection of phosphatase reagents includes the largest collection of human phosphatase expression clones and substrate trapping mutant expression clones.  Largest collection of active phosphatase enzymes, human phosphatase expression clones, and substrate trapping mutant expression clones  Purified GST-tagged active phosphatases for in vitro assay studies  Protein tyrosine, lipid, and dual specificity pho…