Products & ReviewLife Sciences

Rapid Digestion–Trypsin and Rapid Digestion–Trypsin/Lys-C Kits

Promega Corp.VA1060, VA1061

To shorten the time required to prepare samples for LC-MS/MS analysis, we have developed an optimized protocol in which rapid and efficient trypsin digestion is achieved at temperatures as high as 70°C. Using this approach, proteolytis reaction times are dramatically shortened, no chemical denaturants are added and off-line sample cleanup is unnecessary, leading to shorter preparation times and diminished sample losses.

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Description

To shorten the time required to prepare samples for LC-MS/MS analysis, we have developed an optimized protocol in which rapid and efficient trypsin digestion is achieved at temperatures as high as 70°C. Using this approach, proteolytis reaction times are dramatically shortened, no chemical denaturants are added and off-line sample cleanup is unnecessary, leading to shorter preparation times and diminished sample losses.

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Rapid Digestion–Trypsin and Rapid Digestion–Trypsin/Lys-C Kits - Promega Corp. - Life Sciences

Rapid Digestion–Trypsin and Rapid Digestion–Trypsin/Lys-C Kits

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