PCR and Thermal Cycling Products & Reviews

The PX1 PCR plate sealer includes the heat sealing instrument with power cord, a single plate support block that fits both 96- and 384-well plates, and a sealing frame to aid in heat sealing.

Introducing the LightCycler® 96 Real-Time PCR System What if you had the power to get your qPCR data fast, reliably and with less effort? Well, now you can. The result of more than a decade of Roche experience developing high-performance qPCR systems is now accessible for any research lab. The LightCycler® 96 System delivers data that makes you a publication superhero: • Fast precision thermal cycling • Leading-edge optics fo…

The TaqMan® One-Step RT-PCR Master Mix Reagents Kit, which combines the reverse transcription and amplification steps, permits excellent results even when quantifying minute amounts of RNA. Set-up is quick, easy, and minimizes risk of sample crossover and contamination, making this kit well-suited for all high-throughput applications. • Optimized for high sensitivity and yield • Combined RT (reverse transcription) and amplific…

Syzygy FlashTaq 2x Master Mix is a ready-to-use 2x mixture of FlashTaq, dNTPs, buffers and magnesium for efficient PCR amplification. Just add template DNA, primers, and water. FlashTaq Hot-Start DNA Polymerase is a chemically modified Taq polymerase designed for reducing nonspecific DNA amplification including primer dimers. The activiation time for FlashTaq is only 2 minutes at 95°C, which is faster than that for leading a…

rEVAlution 2X qPCR Master Mix is a ready to use hot-start qPCR master mix with EvaGreen® and FlashTaq, a hot start Taq polymerase. A 25μM ROX reference dye is supplied separately for use with some real time instruments. The master mix is optimized to work with both fast cycling conditions and regular three step cycling conditions. Available in 3 pack sizes: 100 20µL Reactions, 500 20µl reactions and 1,000 20µl reactions.

Gel electrophoresis DNA detection

Lateral flow DNA detection

Real-time fluorescent RNA detection

PrimeSTAR Max DNA Polymerase is a unique high fidelity enzyme that is excellent for fast PCR. Conveniently formulated as a 2X premix with reaction buffer and dNTPs, PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any commercially available enzyme, along with extremely high sensitivity, processivity and specificity. It includes an elongation factor to provide efficient priming and extension,…

Primer and probe premixed assays for analyzing gene expression in any species using fluorescently labeled 5' nuclease probes

Rapid and accurate detection of foodborne pathogens

Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…

Droplet Digital PCR System, includes droplet generator, droplet reader, laptop computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications

Obtain high quality results with this thermostable probe-based qPCR master mix

1 ml, one-step RT-PCR kit, includes 2x one-step SYBR Green reaction mix (Taq polymerase, buffers, ROX normalization dyes), iScript reverse transcriptase, and nuclease-free water, for 100 x 20 µl reactions

SensiFAST™ cDNA Synthesis Kit has been developed to generate high-quality first strand cDNA from total RNA template. SensiFAST™ cDNA Synthesis Kit provides a rapid and sensitive method for first strand cDNA synthesis for use in real-time PCR studies (fig. 1). SensiFAST cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in your cDNA template, reg…

SensiFAST™ Probe Hi-ROX Kit and SYBR® Hi-ROX Kit have been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that SensiFAST Kits delivers fast, highly-specific and ultra-sensitive real-time PCR.SensiFAST™ Probe Hi-ROX Kit has been d…

2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 200 x 20 µl reactions

Our comprehensive UltraFlux® PCR offering includes tubes, strips, plates, and sealing film for any experimental need. UltraFlux Tube and Strip Tube Features: Angularly attached caps on the UltraFlux® i and SnapStrip® strip tubes prevent cap lids and hinges from interfering with each other. Ultra-thin walls ensure efficient thermal transfer and thus maximum yield. SSI wall thickness is 0.20mm compared to thicker 0.25mm of…

Verigene® System instrumentation consists of a Verigene Reader and one or multiple Verigene Processor SPs. The Verigene Processor SP is a modular, easy-to-use benchtop analyzer that combines automated nucleic acid extraction, purification, amplification (if required) and hybridization in each module. These advanced capabilities allow users to run Verigene Tests on-demand in response to testing needs, without the need for batc…

SapphireAmp Fast PCR Master Mix is a hot start PCR master mix that contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent in a convenient 2X premix. SapphireAmp PCR Master Mix is especially designed for fast PCR reactions that can be completed in half the time required when using conventional Taq DNA polymerase. For human genomic DNA, amplification of 2 kb targets can be…

PrimeSTAR GXL DNA Polymerase is a high fidelity polymerase that provides efficient PCR amplification even in challenging situations, such as when amplifiying GC-rich templates, when there is eXcess template in the reaction mixture, and when amplifying Long products up to 30 kb (GXL). PrimeSTAR GXL Polymerase is the most robust high fidelity polymerase available, routinely allowing amplification of PCR products that cannot be…

The Takara Bio TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies.

SingleShot™ Probes One-Step Cell Lysis and RT-qPCR Analysis Kit, 100 x 50 µl reactions

Made of thin wall polypropylene and designed for precise fit in heat blocks to optimize heat transfer Nonsterile and certified DNase- and RNase-free Autoclavable at 121°C and will withstand centrifugation to 10,000 x g