PCR and Thermal Cycling Products & Reviews

The DNA kits are ideal for automated sizing and quantitation of PCR fragments, restriction digests or fragmented DNA.

Brilliant III qPCR and QRT-PCRE Master Mixes are more sensitive and faster via the quick-activating hot start and the engineered Taq-mutant. Brilliant III ultrafast maintains amplification efficiency, R2, dynamic range, and detection sensitivity. Shorter total cycle time.

PCR Master Mix(a,b) includes Nuclease-Free Water and PCR Master Mix, 2X. PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. The PCR Master Mix has been optimized for use in routine PCR for amplifying DNA templates in the range of 0.2–2kb.

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. Available with either green or colorless reaction buffers, which also serve as loading buffers, allowing you to go directly from thermal cycler to gel analysis. GoTaq® Hot Start Mast…

Original Eppendorf PCR tubes are manufactured to the highest Eppendorf quality standards. These thin-walled polypropylene tubes ensure efficient and homogeneous heat transfer in the sample thanks to the even wall thickness and smooth wall surface. Heat-sealed bags enclosed in boxes sealed with a PCR Clean sticker ensure the highest degree of purity. A frosted labeling area on the tube allows easy sample tracking

Original Eppendorf PCR tubes are manufactured to the highest Eppendorf quality standards. These thin-walled polypropylene tubes ensure efficient and homogeneous heat transfer in the sample thanks to the even wall thickness and smooth wall surface. Heat-sealed bags enclosed in boxes sealed with a PCR Clean sticker ensure the highest degree of purity.

With its low noise level, the Mastercycler nexus can be used all day in the lab without disturbing any parallel work going on. Additionally, the low power consumption helps to save money and to be eco-friendly. Eppendorf Mastercycler nexus Family In the age of networking, the new Mastercycler nexus is your reliable companion when it comes to the daily routine of PCR. It is easy to use, does not need much space or energy…

 The epMotion 5075 is the ideal solution for diverse liquid handling demands. It offers the same outstanding accuracy and precision as epMotion 5070 and 5073. The available options make the 5075 an excellent and flexible device for applications such as NGS library preparation, real-time PCR set-up, magnetic bead-based and filter-based purification, cell-based assays or any routine pipetting tasks.

The new Mastercycler® X50 elegantly combines speed, flexibility, and ease of use. With its intuitive touch screen offering precise fingertip control, the MC X50 PCR thermocycler enables easy PCR optimization in advanced molecular biology research and dependable standardization in routine PCR applications. It is also perfectly fitted for NGS.

Eppendorf real-time PCR Tube Strips feature an extremely thin wall for optimal heat transfer combined with high mechanical stability. In combination with their optical properties these are the ideal tube strips for any real-time PCR, especially for use with small reaction volumes. The Masterclear™ Cap Strips offer further advantages for your real-time PCR. The inverted dome of the Masterclear™ Cap Strips prevents scratching or…

The Applied Biosystems 7500 Real-Time PCR System is a leading edge system with features to support labs requiring more capabilities from their real time PCR platform. Run time of less than 2 hours Variable excitation and five-color detection enable you to use a broad range of fluorophores including FAM™/SYBR® Green I, VIC®/JOE, NED™/ TAMRA™/ Cy3™, ROX™/Texas Red®, and Cy5™ dyes for greater assay versatility. Measure g…

The Roche LightCycler 480 System - Another breakthrough in real-time PCR analysis of gene expression and melting curve-based mutation analysis.The Roche LightCycler 480 RT-PCR System combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis.This Roche Light Cycler PCR supports all…

Modular thermal cycler platform, includes C1000 Touch™ thermal cycler chassis, CFX96 Deep Well™ optical reaction module, CFX Manager™ software, qbasePLUS software license, cables, reagents, consumables

Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…

The PrimeScript RT-PCR Kit is a two-step RT-PCR kit featuring PrimeScript RTase, an RNase H Minus reverse transcriptase enzyme that offers robust reverse transcription of virtually any RNA template. This RT-PCR kit is conveniently configured to include all reagents needed for two-step endpoint RT-PCR, including primers, PrimeScript RTase, and PCR polymerase enzyme. PrimeScript RTase is an MMLV Reverse Transcriptase and enable…

KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in significant improvements to yield, sensitivity, speed, target length, and the ab…

100 x 20 µl reactions, reverse-transcription reagent kit, includes 5x iScript reaction mix, iScript reverse transcriptase, nuclease-free water

Custom design your oligonucleotide according to your specifications. Our probes and primers are manufactured at unmatched quality under ISO 9001 and 13485 compliance. Whether you need RUO or GMP grade oligos, choose from a vast selection of modifications to customize your oligos for your application.

Software for real-time PCR plate setup, data collection, statistics, and graphing of results

Flexible configuration and control Now cloud-enabled for secure remote access The Applied Biosystems ProFlex PCR System combines the reliability and performance you’ve come to expect from Applied Biosystems thermal cyclers with the flexible configuration and control features that fit how you work today. Interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently. You can…

Flexibility for today, tomorrow, and beyond  

The SimpliAmp™ Thermal Cycler is a small, easy-to-use, and accurate thermal cycler that suits any lab's needs for everyday PCR. It is crafted with the reliable engineering and performance you’ve come to expect from Applied Biosystem® instruments. It is elegantly designed to deliver quiet cycling, and features an innovative, safe heated lid and a large LCD color touch screen. Key Features and Benefits: VeriFlex™ blocks…

Real-time PCR-based gene expression profiling.

SingleShot™ SYBR® Green Cell Lysis and RT-qPCR Kit, 100 x 50 µl reactions

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive