Phusion™ High-Fidelity DNA Polymerase

"Finnzymes' unique Phusion™ High-Fidelity DNA Polymerase offers performance that no other enzyme can match. The Phusion DNA Polymerase generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning and set a new standard for PCR performance. The error rate of Phusion DNA Polymerase (4.4 x 10^-7 when using Phusion™ HF Buffer) is 50-fold lower than that of Thermus aquaticus DNA polymerase, and 6-fold lower than that of Pyrococcus furiosus DNA polymerase - making it the most accurate of available thermostable polymerases.

Incorporating an exciting new technology, Phusion High-Fidelity DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain. By fusing a particular double-stranded DNA binding domain to the polymerase, its processivity can be increased 10-fold. The processivity of Phusion DNA Polymerase is 10-fold greater than that Pyrococcus furiosus DNA polymerase and twice that of Thermus aquaticus DNA polymerase. Phusion High-Fidelity DNA Polymerase exploits this dramatic increase in processivity, resulting in shorter extension times, more robust and high yield amplification, and the ability to amplify long templates in a fraction of the time.