Analyzing T-Cell metabolism using the XF T cell metabolic profiling kit and Seahorse Analytics
2 Aug 2023
In this video, Agilent Technologies showcases its XF T cell metabolic profiling kit and two tailored assays, the XF T cell Persistence Assay and XF T Cell Fitness assay. Using Seahorse Analytics, Agilent demonstrates how to analyze data from these assays. The XF T cell Persistence assay evaluates T cell memory-like phenotype, while the XF T Cell Fitness assay assesses bioenergetic capacity in tumor microenvironment-like conditions. Through spare respiratory capacity and ATP production rate analysis, they identify differences in metabolic profiles between IL-2 and IL-15 expanded cells. The video aims to aid researchers in understanding T cell metabolism for T cell therapy development.
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Seahorse XF T Cell Metabolic Profiling Kit
Agilent TechnologiesThe Agilent Seahorse XF T Cell Metabolic Profiling kit allows for robust and accurate measurements of both glycolytic and mitochondrial activities in T cell populations, providing a complete picture of T cell energy metabolism. These measurements can be linked to antitumor properties of T cell therapy products and are therefore valuable in designing and optimizing therapy development processes to improve T cell persistence or avoid exhaustion in the tumor microenvironment. The kit not only prov
