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A step-by-step workflow for a PDCD1 knock-out experiment in T cells

19 Dec 2023
A step-by-step workflow for a PDCD1 knock-out experiment in T cells

This step-by-step tutorial video demonstrates the process of conducting a PDCD1 gene knock-out in T cells using the Neon™ NxT Electroporation System. See how to design the experiment using the Invitrogen™ TrueDesign™ Genome Editor software; prepare both cells and reagents; conduct post-transfection assessment; and validate the success of the PDCD1 knock-out for further experimentation. For additional support designing CRISPR gene editing experiments, visit thermofisher.com/geneediting or explore the other tutorial videos in this series:

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Gene EditingGene editing is the precise alteration to an organism's DNA, with CRISPR being the most well-known technique. It is used in a wide range of applications, including the development of disease models and gene therapy, and crop improvement. Browse our peer-reviewed product directory to find the best gene editing solutions, compare products, check reviews, and get pricing directly from manufacturers.
A step-by-step workflow for a PDCD1 knock-out experiment in T cells