UHPLC and HPLC

High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

UHPLC and HPLC Webinars

See all

Mastering sorptive extraction for GC–MS

Speakers: Rachael Szafnauer, Markes International Ltd, Olivia Long, SelectScience
21 Jan 2026
Watch on-demand

Why biphenyl is replacing C18 in HPLC

Speakers: Samantha Herbick, Restek Corporation, Olivia Long, SelectScience
18 Nov 2025
Watch on-demand

UHPLC and HPLC Videos

See All