How to Buy HPLC Technology

29 Oct 2025

Cameron Smith-Craig

Pharma and Applied Sciences Editor

High-performance liquid chromatography (HPLC) stands as one of the foremost analytical techniques in use today. Its operational principle involves the separation of compounds according to their polarity. Unlike traditional liquid chromatography (LC), HPLC uses elevated pressure to propel the solvent through the column, significantly expediting separations while minimizing solvent usage.

How-to-buy ebook

Choose the best technology for your laboratory

In this 10th edition eBook, we present an expert guide to help you purchase the most suitable HPLC solution for your work.

Purchasing a new HPLC tool can be a challenging and overwhelming task. It requires careful consideration of various factors, such as the specific analytical needs of the laboratory, budget constraints, available space, and the ever-evolving landscape of technology options. This eBook is here to serve as your guide, providing valuable insights and highlighting key considerations.

Download this SelectScience How to Buy eBook to find out more about:

The principles of HPLC
Sample preparation for HPLC
Top tips for selecting the right column
Determination of separation mode
Column selection by stationary phase
Column selection based on column dimensions
Current hot areas of application

Resource details:

Document type: SelectScience guide
Page count: 21
Read time: 31.5 mins
Edition: 10th

Editors picks

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Raptor™ Biphenyl LC Column
Restek Corp.
With Raptor™ LC columns, Restek chemists became the first to combine the speed of superficially porous particles (also known as SPP or “core-shell” particles) with the resolution of highly selective USLC® technology. This new breed of chromatographic column allows you to more easily achieve peak separation and faster analysis times without expensive UHPLC instrumentation.
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Thermo Scientific™ Vanquish™ Duo UHPLC Systems for Dual LC
Thermo Fisher Scientific
Double your throughput and deepen your sample analysis using the Thermo Scientific Vanquish Duo UHPLC System for Dual LC. Features a dual split sampler and two pumps and flow paths, allowing you to run the same method or two different methods simultaneously on the same instrument, maximizing your lab productivity and return on investment.
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Waters Alliance™ HPLC System
Waters
An integrated HPLC system that works as hard as you do, available with push-button mass detection for the chromatographer. The Alliance™ HPLC System is the flexible and reliable workhorse that meets your fundamental HPLC separation requirements. The system has been continually expanded and perfected to accommodate an enormous range of real-world analytical challenges, culminating in today’s new industrial design which signifies Waters’ commitment to HPLC and our HPLC line. Today, users can extend the Alliance HPLC System’s performance with XP 2.5 µm Columns. XP Columns are compatible with both HPLC and UPLC technology and are designed to not only improve our HPLC productivity, but also facilitate a transfer to UPLC Technology platforms as well. Directly scalable between UPLC and HPLC particle sizes, these new 2.5 um XP Columns bridge the gap between HPLC and UPLC performance. Whether your lab analyzes samples in the pharmaceutical, chemical, food safety, environmental, or quality control arenas, the Alliance HPLC system offers you a field-proven solution. Integrated solvent and sample management to ensure consistent system-to-system performance and high reproducibility. Waters Alliance™ HPLC System Features: Full integration with either Empower® or MassLynx® Software for instrument control and data processing A large, intuitive LCD-based user interface allows rapid system set-up through SystemPREP routines that streamline daily start-ups Easy, tool-free maintenance resulting in maximized uptime Waters complete line of detection technologies – from routine UV/Vis detectors, to the ACQUITY QDa Detector to single, tandem and triple quadrupole mass spectrometers - extend your application capabilities
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SecurityGuard Standard HPLC
Phenomenex Inc
SecurityGuard Guard Cartridge System Column Protection is designed to fit any manufacturers’ standard HPLC column, making it the ultimate HPLC Guard Column. SecurityGuard Guard Cartridge System is guaranteed to extend the lifetime of your 2.0 mm, 3.0 mm, 3.2 mm, 4.6 mm, and 7.8mm ID column by capturing sample contaminants that can cause an increase in backpressure and effect baseline noise. Features: Extends HPLC column lifetime Won’t alter chromatography Compatible with virtually all HPLC columns Easy to determine when to change cartridge Easy to use
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Syringe Filters
Agilent Technologies
Sample filtration is critical to achieving optimal system performance. Agilent Captiva Premium Syringe Filters make the process faster than ever with the industry’s highest flow rates and loading capacities. In addition, Agilent sets the standard for LC/MS testing and certifying more syringe filters to be completely free of observed extractables than any other manufacturer. All Agilent Captiva Premium Syringe Filters are supplied with a HPLC or LC/MS Certificate. Choose from a variety of
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Axcend Focus LC®
Axcend®
The Axcend Focus LC® is a fully functional HPLC system built to deliver dependable, real-time data in a compact form factor.
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