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Rapid Purification of Tocopherols by Flash Chromatography

Teledyne Labs

Rapid Purification of Tocopherols by Flash Chromatography

27 Jul 2015

Tocopherols are fat soluble vitamins with antioxidant activity found in most vegetable oils and in many edible plants. However, analysis and purification of tocopherols presents a challenge due to their relatively low concentration and chromatographic similarity to the oil matrix. This application note describes a single-step method using an automated flash chromatography system to remove the tocopherols from the oil matrix.

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RediSep ® Gold ® ​​ C8​

Teledyne Labs

RediSep Rf Gold C8 reversed-phase columns are packed with 20–40 µm spherical bonded silica, providing improved separation. Achieve near prep-HPLC results with greater sample recovery to easily purify up to gram-scale on your Flash system. Increased pore size optimized for purification of larger molecules like peptides and proteins.

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Flash ChromatographyFlash chromatography is a fast-preparative column chromatography technique used for separation and purification of liquid analytes. Flash chromatography systems use small silica gel particles for separation and a pressurized gas for driving the solvent through the column or pre-packed cartridge. Find the best flash chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Food and Beverage AnalyzersFood and Beverage Analyzers are commonly used to provide information about the chemical composition, structure and physical properties of food materials.Food and Beverage ProcessingFood and Beverage Processing involves the preparation of food products for further analysis. Typical instrumentation includes blanchers, color sorters, cookers, grinders, and refrigeration. These are used to replicate the processes commonly used in the commercial preparation of food and beverages.AntioxidantNatural ProductsFood TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.Olive Oil

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Rapid Purification of Tocopherols by Flash Chromatography