Easy perfusion for anchorage-dependent cell culture

29 Jan 2020

Anchorage-dependent cells, such as Vero cells, are widely used as a platform for viral vaccine production. In perfusion bioprocesses it is possible to constantly add nutrients and to remove byproducts, while retaining the cells in the bioreactor. In this application note, Eppendorf demonstrates how the BioFlo 320 vessel equipped with a spin filter for microcarrier can be used as a platform for high-density Vero cell culture, suitable for vaccine production.

Eppendorf New Brunswick™ BioFlo®/CelliGen® 510

Eppendorf

BioFlo 510 Fermentors and CelliGen 510 Bioreactors are intermediate systems ideal for pilot to small scale production processes. The modular design features a wide variety of standard options allowing this system to be flexible enough to meet most process requirements.   Eppendorf New Brunswick™ BioFlo®/CelliGen® 510 Working volumes: 10.75 L to 32.0 L Applications: Laboratory and pilot scale fermentation of aerobic and anaerobic bacteria, yeasts, and fungi; Laboratory and pilot scale cell culture of mammalian, insect, and human cell lines; Special applications such as stem cell culture, vaccine, monoclonal antibody, or biofuel/biopolymer production; Suitable for batch, fed batch, and continuous processes

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Eppendorf BioFlo® 320

Eppendorf

 The BioFlo 320 seamlessly blends beauty and utility in one all-inclusive package. A new industrial design, flexibility between interchangeable autoclavable and single-use vessels, intelligent sensors, Ethernet connectivity, and a new software package are only a few of the features that set it apart from the competition

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Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BioprocessingBioprocessing is the use of biological systems for the conversion of raw materials to desired products. This includes the research, development and manufacturing stages of production and can refer to food processing, small-molecule pharmaceutical manufacture, production of recombinant protein therapeutics, or the generation of renewable energy.Vaccine DevelopmentVaccine development focuses on creating immunizations to protect against infectious diseases. The process involves identifying antigens, developing and testing vaccine efficacy, and ensuring safety through preclinical testing and multiple clinical trial phases. Scientists use various approaches, including weakened or inactivated viruses, protein subunits, or mRNA. Advanced platforms and rapid diagnostic tools aid in vaccine research and testing. Explore vaccine development solutions in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.
Easy perfusion for anchorage-dependent cell culture