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Determination of Mycotoxin Residues by LC-MS/MS Featuring Two Alternate Sample Extraction Procedures
20 Apr 2016Mycotoxins are toxic natural secondary metabolites and are produced by many species of fungi, while over 300 mycotoxins have been identified only a few of these pose a significant food or animal feed safety risk. They are still of concern however as they cannot be destroyed during food processing and heat treatment, therefore it is important that food can be analyzed to detect their presence. This application note describes a method to detect mycotoxins using LC-MS/MS with two different sample preparation steps.
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SELECTRA® HPLC Columns
UCT, Inc.High purity, Type B, spherical silica support. 100A pore size. 1.8um, 3um, & 5um partical sizes. Variety of stationary phases.
ChloroFiltr® for QuEChERS dSPE Clean-Up
UCT, Inc.Chlorophyll in QuEChERS extracts must be removed to reduce potential instrument downtime. This is removed from extracts using graphitized carbon black (GCB) Unfortunately, GCB also removes planar analytes reducing analytical accuracy. ChloroFiltr®, used in place of GCB in dSPE tubes, removes unwanted chlorophyll without removing pesticides or environmental analytes. No change in procedure are required.
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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.Robotic InstrumentsRobotic instruments can be used for high-throughput automation of many lab processes. Such processes use instruments for assays like cell based assays and ELISA, for sample preparation like shakers, centrifuges and incubators, and for analysis such as sequencing and western blot analyzers. Useful features of robotic instruments include speed, reproducibility, barcode readers, software and automation.Food and Beverage AnalyzersFood and Beverage Analyzers are commonly used to provide information about the chemical composition, structure and physical properties of food materials.Food and Beverage ProcessingFood and Beverage Processing involves the preparation of food products for further analysis. Typical instrumentation includes blanchers, color sorters, cookers, grinders, and refrigeration. These are used to replicate the processes commonly used in the commercial preparation of food and beverages.QuEChERSThe QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method is a streamlined approach that is used to extract multi-residue target compounds from food samples. It is most commonly used by analytical chemists studying pesticide residues in food.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Contamination PreventionContamination prevention aims to control and minimize the risk of contamination during experiments. Biological safety cabinets and sterilization equipment are examples of technologies used to prevent contamination.Food SafetyFood safety describes the prevention of food-borne illnesses. Contamination with a variety of pathogens can be prevented with adequate temperature control, hygiene and labeling. Food samples can be tested using GC/MS, HPLC, SPE, and qPCR to ensure their adherence to regulations.MycotoxinsToxic chemicals such as Aflatoxin and Alkaloids that are produced by fungi are known as mycotoxins. In humid conditions, fungi can proliferate and colonize on crops to levels that can be damaging to human health. Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.Food ContaminationFood contamination refers to the presence of harmful substances or pathogens in food, posing risks to human health. Contaminants can be biological, chemical, or physical, and their detection is crucial for ensuring food safety. Advanced testing methods like PCR, chromatography, and spectroscopy are used to identify contaminants in food products. Browse our peer-reviewed product directory to find the best solutions for food contamination detection, compare products, check reviews, and get pricing directly from manufacturers.Solid Phase Extraction (SPE)Solid phase extraction (SPE) is a sample preparation technique used to separate and concentrate analytes from complex mixtures. It is widely used in environmental, pharmaceutical, and clinical analysis. SPE provides higher sensitivity and selectivity compared to traditional liquid-liquid extraction methods, making it essential for accurate and reproducible results. Explore SPE tools and systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.