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Determination of High Sugar Concentrations in an Irish Whiskey Sample Using a Compact Ion Chromatography System

25 May 2016

In this application note a HPAE-PAD method is used to determine the glucose, fructose and sucrose concentrations in a 5-fold diluted Irish whiskey sample. To increase the sensitivity this method uses the Thermo Scientific™ Dionex™ Integrion™ RFIC™ system with a 0.4 μL internal injection loop and the Thermo Scientific™ High Concentration Carbohydrate Analysis Kit1 to extend the linearity from low mg/L to g/L concentrations.

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Thermo Scientific™ Dionex™ Integrion™ HPIC™ System

Thermo Fisher Scientific

As an ion chromatographer, the routine IC applications you run give the rest of us the peace of mind to use everyday resources like water, food and beverages, biofuels and pharmaceuticals without a second thought. Using this high-pressure-capable IC system to separate and quantify common ionic pollutants in municipal drinking water and wastewater can provide faster, more cost-effective methods for regulatory compliance testing.   Nothing supports you in this role more than your laboratory instruments. The Thermo Scientific™ Dionex™ Integrion™ HPIC™ system is designed to be your reliable lab partner.     Features:   Simplified plumbing layout with integrated architecture - Layout based on logical flow-path for easy component access in a compact platform with a small physical footprint. Consumables Device Monitor - Provides positive identification of a consumable's performance metrics, preventing consumable installation errors and mismatches by automatically logging consumable use and performance. Detachable tablet with local language support - Flexible access to IC controls that speak your language Upgradeable features - In a fluid market with changing demands, the Dionex Integrion IC system offers a wide range of upgradable components, so it can grow with your needs over time. These include electrolytic sample preparation and electrolytic water purification Automated Eluent Generation (EG™) - Eliminate human errors associated with manual eluent preparation and improve method reproducibility from operator-to-operator and lab-to-lab. The Dionex Integrion IC system brings consistent results to all your applications. In this case, 100 injections of a simple sugar mixture are highly reproducible. Wide range of column support - Choose the column sizes you need for your applications, including smaller-particle-size 4 µm columns that provide faster run times and better resolution. Flexibility – two different models to choose from and option to choose right detector or column format for your application

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AutosamplersAutosamplers are automated sample loaders used with a number of separation and analytical techniques. HPLC autosamplers use automated partial or full-loop injection systems and place samples in vials or microplates for analysis. Autosamplers can also be used for elemental analysis. Features to consider include repeatability, syringe capacity, footprint and temperature controls.  Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. 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Typical instrumentation includes blanchers, color sorters, cookers, grinders, and refrigeration. These are used to replicate the processes commonly used in the commercial preparation of food and beverages.GlucoseChromatography Method DevelopmentChromatography method development is the process of designing and optimizing chromatographic techniques to separate and analyze complex mixtures. This includes selecting appropriate stationary and mobile phases, determining flow rates, and optimizing detection methods. Successful method development is essential for high-quality results in fields like pharmaceuticals, food safety, and environmental analysis. Explore chromatography method development tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Food SafetyFood safety describes the prevention of food-borne illnesses. Contamination with a variety of pathogens can be prevented with adequate temperature control, hygiene and labeling. Food samples can be tested using GC/MS, HPLC, SPE, and qPCR to ensure their adherence to regulations.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. 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