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Determination of High Sugar Concentrations in a Flavored Rum Liquor Sample Using a Compact Ion Chromatography System

19 May 2016

In this application note the concentrations of glucose, fructose and sucrose are determined 100-fold diluted flavored rum alcohol sample by HPAE-PAD. The technique in this application note uses a Thermo Scientific™ Dionex™ Integrion™ RFIC™ system equipped with a 0.4 μL internal injection loop and the Thermo Scientific™ High Concentration Carbohydrate Analysis Kit1 which extends the linearity from low mg/L to g/L concentrations.

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Thermo Scientific™ Dionex™ Integrion™ HPIC™ System

Thermo Fisher Scientific

As an ion chromatographer, the routine IC applications you run give the rest of us the peace of mind to use everyday resources like water, food and beverages, biofuels and pharmaceuticals without a second thought. Using this high-pressure-capable IC system to separate and quantify common ionic pollutants in municipal drinking water and wastewater can provide faster, more cost-effective methods for regulatory compliance testing.   Nothing supports you in this role more than your laboratory instruments. The Thermo Scientific™ Dionex™ Integrion™ HPIC™ system is designed to be your reliable lab partner.     Features:   Simplified plumbing layout with integrated architecture - Layout based on logical flow-path for easy component access in a compact platform with a small physical footprint. Consumables Device Monitor - Provides positive identification of a consumable's performance metrics, preventing consumable installation errors and mismatches by automatically logging consumable use and performance. Detachable tablet with local language support - Flexible access to IC controls that speak your language Upgradeable features - In a fluid market with changing demands, the Dionex Integrion IC system offers a wide range of upgradable components, so it can grow with your needs over time. These include electrolytic sample preparation and electrolytic water purification Automated Eluent Generation (EG™) - Eliminate human errors associated with manual eluent preparation and improve method reproducibility from operator-to-operator and lab-to-lab. The Dionex Integrion IC system brings consistent results to all your applications. In this case, 100 injections of a simple sugar mixture are highly reproducible. Wide range of column support - Choose the column sizes you need for your applications, including smaller-particle-size 4 µm columns that provide faster run times and better resolution. Flexibility – two different models to choose from and option to choose right detector or column format for your application

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AutosamplersAutosamplers are automated sample loaders used with a number of separation and analytical techniques. HPLC autosamplers use automated partial or full-loop injection systems and place samples in vials or microplates for analysis. Autosamplers can also be used for elemental analysis. Features to consider include repeatability, syringe capacity, footprint and temperature controls.  Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.Robotic InstrumentsRobotic instruments can be used for high-throughput automation of many lab processes. Such processes use instruments for assays like cell based assays and ELISA, for sample preparation like shakers, centrifuges and incubators, and for analysis such as sequencing and western blot analyzers. Useful features of robotic instruments include speed, reproducibility, barcode readers, software and automation.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. Find the best robotic workstations in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Food and Beverage AnalyzersFood and Beverage Analyzers are commonly used to provide information about the chemical composition, structure and physical properties of food materials.Food and Beverage ProcessingFood and Beverage Processing involves the preparation of food products for further analysis. Typical instrumentation includes blanchers, color sorters, cookers, grinders, and refrigeration. These are used to replicate the processes commonly used in the commercial preparation of food and beverages.Particle CharacterizationParticle characterization instruments are used to determine particle size distribution, shape, surface area, zeta potential, density and porosity of particles and materials. Multiple tecchniques are available for determining particle size, shape and count including dynamic light scattering (DLS), laser diffraction, electrozone (Coulter technique), imaging particle analysis and single particle optical sensing. Determine the density of your material with a gas pycnometer or examine its surface area and porosity with gas adsorption analyzers and mercury porosimeters. Find the best particle characterization instruments in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.GlucoseMethod DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.CarbohydratesAlcoholic BeveragesThe analysis of alcoholic beverages involves testing their composition, quality, and safety, including alcohol content, flavor compounds, and impurities. This is essential for quality control and regulatory compliance. Explore tools for alcoholic beverage testing in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Determination of High Sugar Concentrations in a Flavored Rum Liquor Sample Using a Compact Ion Chromatography System