ResourceDrug discovery > Basic Research
Batch Immobilization of a Biotinylated Ligand onto Streptavidin Biosensors
8 Jun 2017This technical note outlines a general batch mode procedure for immobilizing a biotinylated ligand onto Streptavidin biosensors in the biosensor tray assembly on the benchtop. The goal is to develop a procedure that creates a binding surface having a maximum and reproducible response. The protocol outlines determining the optimal concentration and time for immobilization on-line and then the transfer of these parameters to off-line immobilization.
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