ResourceSeparations

Acquity UPLC Analysis of Edible Oils

3 Jul 2016

This application note describes a 10-minute separation and identification method for analysis of edible oils with Waters® ACQUITY UPLC®/PDA system using low toxic solvents, acetonitrile and 2-propanol as the mobile phase. The UPLC/PDA method not only allows faster and more precise analysis of triglycerides to authenticate the edible oils but also detects oxidized and decomposed triglyceride, enabling simultaneous determination of oil purity.

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AutosamplersAutosamplers are automated sample loaders used with a number of separation and analytical techniques. HPLC autosamplers use automated partial or full-loop injection systems and place samples in vials or microplates for analysis. Autosamplers can also be used for elemental analysis. Features to consider include repeatability, syringe capacity, footprint and temperature controls.  UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. 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