A guide to Pichia pastoris fermentation in single-use stirred-tank bioreactors

27 May 2021

In this protocol, Eppendorf describes the detailed procedure of Pichia pastoris fermentation in a BioBLU ®3f Single-Use Bioreactor controlled by a BioFlo® 320 bioprocess control system.

Eppendorf BioFlo® 320

Eppendorf

 The BioFlo 320 seamlessly blends beauty and utility in one all-inclusive package. A new industrial design, flexibility between interchangeable autoclavable and single-use vessels, intelligent sensors, Ethernet connectivity, and a new software package are only a few of the features that set it apart from the competition

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Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.BioreactorBioprocessingBioprocessing is the use of biological systems for the conversion of raw materials to desired products. This includes the research, development and manufacturing stages of production and can refer to food processing, small-molecule pharmaceutical manufacture, production of recombinant protein therapeutics, or the generation of renewable energy.
A guide to Pichia pastoris fermentation in single-use stirred-tank bioreactors