PCR and Thermal Cycling > PCR Mastermixes and Kits Products & Reviews

The DNA kits are ideal for automated sizing and quantitation of PCR fragments, restriction digests or fragmented DNA.

PCR Master Mix(a,b) includes Nuclease-Free Water and PCR Master Mix, 2X. PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. The PCR Master Mix has been optimized for use in routine PCR for amplifying DNA templates in the range of 0.2–2kb.

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. Available with either green or colorless reaction buffers, which also serve as loading buffers, allowing you to go directly from thermal cycler to gel analysis. GoTaq® Hot Start Mast…

Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…

KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in significant improvements to yield, sensitivity, speed, target length, and the ab…

The Streck ARM-D Kit, OXA is a multiplex real-time PCR kit for the rapid detection of antibiotic resistance genes in Gram-negative bacteria. This comprehensive kit allows you to detect over 340 allelic variants within 6 OXA gene families, OXA-23, OXA-24/40, OXA-48, OXA-51, OXA-58 and OXA-143.

Smart One RT-PCR Master Mix is designed for all applications that require Reverse Transcription of RNA into cDNA and a subsequent PCR amplification. Only primers and sample RNA have to be added to the mix

PCRBIO Taq DNA Polymerase is an affordable, versatile and robust enzyme for all your everyday PCR applications including genotyping, screening and library construction. An enhanced 12-step purification strategy together with an optimised buffer system enable PCRBIO Taq DNA Polymerase to amplify with the highest speed, yield and specificity.

Thermo Scientific DreamTaq Green PCR Master Mix is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl2, and dNTPs.

PCRBIO Taq Mix Red is an affordable, versatile and robust 2x mix for all your everyday PCR applications including genotyping, screening and library construction. The mix contains a red dye allowing reactions to be directly loaded onto agarose gels.

PCRBIO Classic Taq is a highly purified recombinant Taq DNA Polymerase for all your everyday PCR applications including genotyping, screening and library construction. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA.

A qPCR master mix containing Evagreen qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.                  

FastStart PCR Master is a ready-to-use, 2x-concentrated hot start master mix. It contains all the reagents (except PCR primers and template) required for running standard PCR and two-step RT-PCR on thermal block cycler instruments. Hot start PCR High-throughput PCR Benefits Maximize convenience. All you need to provide is primers, template, and water – the double-concentrated master mix contains everything else you…

"Finnzymes' unique Phusion™ High-Fidelity DNA Polymerase offers performance that no other enzyme can match. The Phusion DNA Polymerase generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning and set a new standard for PCR performance. The error rate…

Applied Biosystems GeneAmp® Fast PCR Master Mix's sensitive, pre-mixed hot-start chemistries reduce PCR reaction time to as little as 25 minutes when used with the 9800 Fast PCR System. • Pre-mixed hot-start chemistry enables you to set up your experiments quickly. • Detect a single copy of a gene in as little as 10 ng of DNA. • Get reproducible, specific, and sensitive results.

PCRBIO HS Taq Mix Red is an advanced antibody-mediated hot start DNA polymerase mix designed for fast, highly specific and sensitive PCR. The mix contains a red dye allowing reactions to be directly loaded onto agarose gels without additional loading buffer. Whether you need a hot start assay for high-throughput or the detection of a low copy number template, PCR Biosystems offers you a robust industry-leading 2x mix.

Eliminate the need for time-consuming DNA extraction methods with this simple, integrated extraction and amplification kit powered by the latest advances in hot start polymerase technology. PCRBIO Rapid Extract PCR Kit combines rapid DNA extraction with fast, highly specific DNA amplification in a convenient, easy-to-use format. Generate PCR-ready DNA in 15 minutes, without spin columns or hazardous chemicals.

The GeneMorph II Random Mutagenesis Kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis. 

The Agilent range of HotStart polymerases and master mixes features Hotstart Taq DNA Polymerase, Methylation Bi-sulfite sequencing, uracil-sensitive solutions and more. 

First generation Pfu polymerases, such as cloned Pfu, PfuTurbo, and PfuUltra, have been improved upon. The improved products, PfuUltra II and Herculase II provide all the benefits of the first generation enzymes plus additional features such as speed to results and larger amplicons.  Cloned Pfu DNA Polymerases  First generation     PfuUltra    DNA Polymerases     PfuTurbo    DNA Polymerases

The engineered PfuUltra II Fusion HS DNA Polymerase provides the worlds most accurate copies and the fastest extension times possible. PfuUltra II Fusion HS DNA Polymerase is PfuUltra hotstart DNA polymerase coupled with a fusion polymerase technology and ArchaeMaxx PCR enhancing factor.  The industry standard for ultra high fidelity PCR providing the most accurate copies of DNA  Dramatically reduced extension times allow f…

The PicoMaxx high fidelity PCR system reliably produces high PCR product yields on a wide variety of templates up to 10 kb. It also provides superior sensitivity by successfully amplifying samples where starting material is limited.  High sensitivity provides maximum PCR reliability  Consistent, robust amplification up to 10 kb  Superior efficiency and yield with our patented ArchaeMaxx polymerase-enhancing factor  Opti…

The PicoMaxx high fidelity PCR system reliably produces high PCR product yields on a wide variety of templates up to 10 kb. It also provides superior sensitivity by successfully amplifying samples where starting material is limited.  High sensitivity provides maximum PCR reliability  Consistent, robust amplification up to 10 kb  Superior efficiency and yield with our patented ArchaeMaxx polymerase-enhancing factor  Opti…

PicoMaxx High Fidelity PCR Master Mix possesses the sensitivity to detect low copy number targets. Plus, it has the robustness to withstand effects of PCR inhibitors without the need for extensive purification and subsequent DNA loss. PicoMaxx reliably produces high PCR product yields on a wide variety of templates up to 10 Kb.