PCR and Thermal Cycling PCR Mastermixes and Kits Products & Reviews

The DNA kits are ideal for automated sizing and quantitation of PCR fragments, restriction digests or fragmented DNA.

The SureDirect Blood PCR Kit enables direct DNA amplification from many sample types including: liquid blood, dried blood on paper, serum, and plasma. Inhibitor resistance and throughput, common PCR challenges associated with blood samples, are averted through the activity of a newly engineered Taq.

The Streck ARM-D Kit, β-Lactamase is a multiplex real-time PCR kit for the rapid detection of β-lactam antibiotic resistance genes in Gram-negative bacteria. This comprehensive kit allows you to detect over 450 allelic variants in 9 clinically relevant carbapenemases, extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated ampC gene families, KPC, NDM, OXA-48, IMP, VIM, DHA, CMY-2, CTX-M-14 and CTX-M-15. 

The Streck ARM-D Kit, OXA is a multiplex real-time PCR kit for the rapid detection of antibiotic resistance genes in Gram-negative bacteria. This comprehensive kit allows you to detect over 340 allelic variants within 6 OXA gene families, OXA-23, OXA-24/40, OXA-48, OXA-51, OXA-58 and OXA-143.

A qPCR master mix containing Evagreen qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.                  

PCR Master Mix(a,b) includes Nuclease-Free Water and PCR Master Mix, 2X. PCR Master Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. The PCR Master Mix has been optimized for use in routine PCR for amplifying DNA templates in the range of 0.2–2kb.

The PowerPlex® Fusion System is a 24-locus multiplex for human identification applications including forensic analysis, relationship testing and research use.   The PowerPlex® Fusion System provides all of the materials needed for co-amplification and five-color fluorescent detection of 24 loci (23 STR loci and Amelogenin), including the CODIS core loci and the European Standard Set (ESS) loci. With 24 loci, the sys…

Process swabs and storage card punches for direct amplification.

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The system is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so tha…

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. Available with either green or colorless reaction buffers, which also serve as loading buffers, allowing you to go directly from thermal cycler to gel analysis. GoTaq® Hot Start Mast…

Takara produce a host of products for PCR Takara’s new sensitive qPCR premixes contains TLi RNase H and are developed to address the inhibitory effect on the PCR due to the presence of remaining RNA after cDNA synthesis. PrimeSTAR®, a non-Taq polymerase with high proofreading activity are now available in a high speed version, PrimeSTAR Max, with extension rate of less than 5 sek/kb and PrimeSTAR GXL, that amplifies up to 30 k…

Applied Biosystems GeneAmp® Fast PCR Master Mix's sensitive, pre-mixed hot-start chemistries reduce PCR reaction time to as little as 25 minutes when used with the 9800 Fast PCR System. • Pre-mixed hot-start chemistry enables you to set up your experiments quickly. • Detect a single copy of a gene in as little as 10 ng of DNA. • Get reproducible, specific, and sensitive results.

Extensor Long Range Enzyme Blend is an optimized mix of ThermoPrime* DNA Polymerase and a proprietary thermostable proofreading enzyme. The two enzymes act synergistically to generate long PCR products, up to 20kb. This makes the enzyme blend ideal for robust full-length amplification of long range sequences. ■ Amplifies targets up to 20kb in length ■ Unique enzyme blend and optimized buffer combination delivers increased accu…

Syzygy FlashTaq 2x Master Mix is a ready-to-use 2x mixture of FlashTaq, dNTPs, buffers and magnesium for efficient PCR amplification. Just add template DNA, primers, and water. FlashTaq Hot-Start DNA Polymerase is a chemically modified Taq polymerase designed for reducing nonspecific DNA amplification including primer dimers. The activiation time for FlashTaq is only 2 minutes at 95°C, which is faster than that for leading a…

Gel electrophoresis DNA detection

PrimeSTAR Max DNA Polymerase is a unique high fidelity enzyme that is excellent for fast PCR. Conveniently formulated as a 2X premix with reaction buffer and dNTPs, PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any commercially available enzyme, along with extremely high sensitivity, processivity and specificity. It includes an elongation factor to provide efficient priming and extension,…

Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…

Obtain high quality results with this thermostable probe-based qPCR master mix

SapphireAmp Fast PCR Master Mix is a hot start PCR master mix that contains a hot start PCR enzyme, optimized buffer, dNTP mixture, gel loading dye (blue), and a density reagent in a convenient 2X premix. SapphireAmp PCR Master Mix is especially designed for fast PCR reactions that can be completed in half the time required when using conventional Taq DNA polymerase. For human genomic DNA, amplification of 2 kb targets can be…

PrimeSTAR GXL DNA Polymerase is a high fidelity polymerase that provides efficient PCR amplification even in challenging situations, such as when amplifiying GC-rich templates, when there is eXcess template in the reaction mixture, and when amplifying Long products up to 30 kb (GXL). PrimeSTAR GXL Polymerase is the most robust high fidelity polymerase available, routinely allowing amplification of PCR products that cannot be…

KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in significant improvements to yield, sensitivity, speed, target length, and the ab…

Platinum® Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzymePyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology.   Features:…

The ZymoBIOMICS™ PCR PreMix is supplied as a 2X concentrated “master mix" and contains all the reagents needed to perform PCR and other molecular downstream analysis with the addition of probes or fluorescent dyes.   It features a hot-start DNA polymerase and is validated low bio burden in regards to bacterial contamination. Simple and easy to use, just add water, primers, and template DNA to the ZymoBIOMICS™ PCR P…

TaKaRa Taq DNA Polymerase and Premix Taq DNA Polymerase

A High Fidelity PCR Enzyme for Long Range PCR: LA Taq DNA Polymerase