KAPA HiFi PCR Kits
KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in significant improvements to yield, sensitivity, speed, target length, and the ab…
Achieve the highest fidelity
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Kapa - Worth trying it once for your complex custom PCR projects
Molecular Biology - PCR
It's a novel B-family DNA polymerase, specifically engineered to increased it's affinity for DNA without accessing accessory proteins or binding domains. We used to have many custom projects with different gene targets of various sizes and multiple gene panels. We had to switch from 5-6 different polymerase to this Kappa enzyme. Now for most of our projects Kappa is the first and only choice The results are amazing both on gel (quality) and quantity (ND/qubit). The concentration was good enough to use for other downstream applications. You don't need to do many trials while using this polymerase enzyme. It's quite stable and easy to set-up any PCR evading complexity of PCR program. It may be a bit costly but the price is worth for the value of product
Review Date: 4 Mar 2023 | Kapa Biosystems, Inc.
KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends.
KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi results in significant improvements to yield, sensitivity, speed, target length, and the ability to amplify difficult templates (e.g AT- and GC-rich). The error rate of KAPA HiFi PCR Kits is 100X lower than wild-type Taq DNA polymerase.
Features:
Achieve the highest fidelity
- Increased processivity, strong proofreading activity, and optimized buffer system result in superior accuracy
- The error rate of KAPA HiFi is 100X lower than Taq, 40X lower than polymerase blends, 3X lower than Pfu Ultra and 2X lower than Phusion
Improve performance on GC- and AT-rich templates
- Achieve higher success rates across targets up to 84% GC content
- Higher yields when amplifying AT-rich templates
Amplify longer targets with greater sensitivity
- High-fidelity PCR of long and complex genomic templates
- Improved sensitivity
- High speed allows significantly shorter reaction times for long range PCR
Applications:
- High-fidelity PCR
- Amplification of DNA fragments for cloning and protein expression or genomic characterization
- Site-directed mutagenesis
- Next-generation sequencing library amplification
