CFX96 Touch™ Deep Well Real-Time PCR Detection System with Starter Package
Modular thermal cycler platform, includes C1000 Touch™ thermal cycler chassis, CFX96 Deep Well™ optical reaction module, CFX Manager™ software, qbasePLUS software license, cables, reagents, consumables
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Great equipment, good results!
Extraction of nucleic acids
High quality and reproductible results, easy to use, good equipment!
Review Date: 20 Feb 2024 | Bio-Rad
Great results... instrument through put extremally good
Analysis of samples for Covid
Very accurate results for large number of batch samples
Review Date: 21 Nov 2023 | Bio-Rad
Easy to use Bio-Rad PCR machine, and help in getting the results easily.
Use for getting PCR products of the sample
Yes, this product is good and easy to handle which give us good results.
Review Date: 15 Jul 2022 | Bio-Rad
Biorad CFX96 is a great qRT-PCR system
qRT-PCR
The system runs well and we can run up to 96 samples per run which is wonderful.
Review Date: 29 Mar 2022 | Bio-Rad
Very good instrument. Use Bio-Rad and work happily.
Analysis of infectious diseases, covid
Bio-Rad CFX96 Real time PCR System is very easy to use. The analysis can be done very effectively with few errors. A new user can also use and understand the system easily. The plates are also of a very good quality. We always prefer Bio-Rad PCR system for use.
Review Date: 26 Jul 2021 | Bio-Rad
Excellent product. Very reliable.
Real time PCR
Excellent product. Very reliable. I use it for all my qPCR analysis. Would definitely recommend. Although the machine is old, but still provides very reliable data.
Review Date: 5 Nov 2018 | Bio-Rad
It is easy to use and I would like to try using it for multiplex assays in environmental bacterial isolates to check for gene expression.
Review Date: 16 May 2014 | Bio-Rad
PCR
As a neophyte in real time PCR, I found this instrument easy to use with excellent phone support from the vendor. Furthermore, the training that came bundled with the instrument was invaluable.
Review Date: 22 May 2013 | Bio-Rad
Pharmacology
This is a good instrument and provides fast measurements, but the software could be simpler to use. Also if it was upgraded with some more applications then it would be good value for money.
Review Date: 3 Jan 2013 | Bio-Rad
Use the CFX96 Touch Deep Well Touch Real-Time PCR Detection System for precise, reproducible real-time PCR. The system is composed of the CFX96 Deep Well Optical Reaction Module, the C1000 Touch Thermal Cycler Chassis, SYBR® Green Supermix, 96-well PCR plates, and plate seals.
The module is a member of the family of interchangeable reaction modules that can be used with the C1000 Touch Thermal Cycler Cchassis for PCR and real-time PCR. All modules can be quickly inserted and removed from the cycler chassis without tools.
Features and Benefits
- Deep wells for large volumes
- Detection of up to 5 targets per well, plus a channel dedicated to singleplex FRET
- Protocol autowriter that generates an optimal protocol for your reaction components
- Thermal gradient that identifies optimal annealing temperature in a single run
- CFX Maestro Software and qbase+ Software for reliable validation, data analysis, and export of RDML files for conformance with MIQE guidelines
The CFX96 Deep Well Real-Time PCR Detection System has six independently controlled thermal electric units providing even, precisely controlled temperatures at all times during the run, including ramping. The reduced mass of the honeycomb block provides fast ramping and reduced settling time (time to achieve thermal uniformity).
The optical system, with six filtered LEDs and six filtered photodiodes, collects data from all wells, detecting up to five targets per well. For single-color FAM and SYBR® Green I, the fast scan option reads single-channel fluorescence in all 96 wells in just 3 seconds. A channel with an LED filter–photodiode combination is dedicated to FRET singleplex experiments.
The large touch-screen interface has intuitive graphical programming and displays data traces in real time. The programmable thermal gradient feature can test eight temperatures at once to quickly determine the optimal annealing temperature, and the protocol autowriter quickly optimizes reactions, generating an optimal protocol based on your polymerase, primers, and product length.
Product Components
- CFX96 Dee Well Optical Reaction Module ( 1840197 )
- C1000 Touch Thermal Cycler Chassis ( 1841100 )
- USB cable
- CFX Maestro Software
- Instruction manual
- qbase+ Software license
- SsoAdvanced™ Universal SYBR Green Supermix ( 1725270 )
- Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates ( HSP9601 )
- Microseal® 'B' Adhesive Seals ( MSB1001 )
Configuration Options
The following modules can be used with the C1000 Touch Thermal Cycler Chassis:
*Starter package contains SsoAdvanced Universal SYBR Green Supermix ( 1725270 ), Microseal 'B' Adhesive Seals ( MSB1001 ), and Hard-Shell Low-Profile Thin-Wall 96-Well Skirted PCR Plates ( HSP9601 ) or Hard-Shell Thin-Wall 384-Well Skirted PCR Plates ( HSP3805 ).
The CFX96 Deep Well Real-Time PCR Detection System can be configured with other PCR instruments, allowing stand-alone or software control. Instrument permutations allow up to four different CFX Connect™, CFX96, or CFX384 Systems to be controlled by one PC.
Combine any two CFX Real-Time PCR Detection Systems with the CFX Automation System II , our robotic plate handler, to create a high-throughput, automated real-time PCR system with a compact footprint.
Service Plans and Validation Tools for CFX Systems
- Ensure that your real-time PCR systems are functioning optimally with Bio-Rad Expert Care Service ; choose from several service plans to keep your CFX Automation System II up and running its best
- Certify system installation and validate system performance with CFX System IQ/OQ Services and CFX Automation System II IQ/OQ Services
- Verify the performance of your CFX Systems with Thermal Validation Services and the CFX Qualification Plates
Related Products
- PX1™ PCR Plate Sealer
- PCR plastics optimized for CFX Real-Time PCR Detection Systems:
- Hard-Shell 96-Well Low Profile Skirted PCR Plates including barcoded plates ( HSP9955 )
- Microseal 'B' Adhesive Seals ( MSB1001 )
- Optically Clear Heat Seals ( 1814030 )
- CFX Maestro Software, Security Edition , for compliance with U.S. FDA 21 CFR Part 11 regulations
- CFX Qualification Plate for performance verification, 96-well ( 1845098 )
Related Categories
- Real-Time PCR Detection Systems
- PCR Reagents and qPCR Reagents
- PCR Plastic Consumables
- PCR Seals
- PCR Analysis Software
Online Resources
- Real-Time PCR Detection Systems Selection Chart
- qPCR/Real-Time PCR
- qPCR Instrumentation
Real-Time qPCR as a Tool for Evaluating RNAi-Mediated
Real-time quantitative PCR (rt-qPCR) is the method of choice for accurate, sensitive, and specific quantitation of
nucleic acid sequences. This report describes how rt-qPCR can be implemented as a tool for monitoring silencing efficiency and assessing functional effects of RNA interference (RNAi)-mediated knockdown of MYCN and TP53 genes, which are pivotal in neuroblastoma pathogenesis.
Relative Quantitation of mRNA: Real-Time PCR vs. End-Point PCR
This application note from Bio-Rad compares the use of end-point PCR and real-time PCR as methods of relative quantitation for viral gene expression. The chosen viral genes used in this study are representative of each phase of herpes simplex virus type 1 (HSV-1) replication.
Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient
This tech report focuses on the use of the thermal gradient feature on the CFX96™ real-time PCR detection system to optimize reverse transcription (RT) and fast PCR conditions for a four-target multiplex PCR assay. It is demonstrated that the gradient feature of the CFX96 system dramatically accelerates the optimization of the RT and PCR steps for multiplexing RT-qPCR assays and improves the ease of converting cycling conditions from a three-step to a two-step PCR assay.
High Resolution Melt Parameter Considerations for Optimal Data Resolution
The popularity of high resolution melt (HRM) analysis has grown considerably in the last few years and HRM analysis now encompasses applications including single nucleotide polymorphism (SNP) analysis, mutation scanning, DNA methylation analysis and species identification. This study analyses the steps performed by HRM software to identify thermal profile differences and examines the effect of assay optimization techniques.
Optical Design of CFX96™ Real-Time PCR Detection System
In contrast to the optical design of most real-time PCR systems, the CFX96 and CFX384™ real-time PCR detection systems utilize solid-state optical technology with long-lasting light-emitting diodes for fluorescence excitation and photodiodes for detection. Therefore eliminating the need to sacrifice data collection in one of the channels to normalize to a passive reference. In this study, evidence is presented to support the methodology and show the impact of reaction volume of fluorescence chemistry on replicate samples.
Touch the Future of PCR - Introducing the 1000-Series Touch Thermal Cyclers
Watch this video to find out how Bio-Rad thermal cyclers offer a user-friendly interface with the reliability and performance that you expect from Bio-Rad. Thermal gradient technology makes it easy to optimize your reactions in a single run. The 96-well sample block and self-adjusting heated lid accommodate standard tubes, tube strips, and plates.
