Cell-Based Assays > Cell Signaling Assays Products & Reviews

Live-cell analysis system

ARRALYZE Glass Nanowell Arrays feature precisely engineered nanowells in borosilicate glass, created with our patented LIDE technology. Each array contains up to 897 individual wells (0.4 × 0.4 × 0.225mm) with dimpled bottoms for optimal single-cell isolation and analysis.

Founded on a growing portfolio of purified lectins, lectin conjugates, and lectin screening kits, Vector Laboratories supports the ability to profile, characterize, and capture complex glycans in biological systems.

Detection of binding partners for 100 different glycans in purified proteins, serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.

Detection of binding partners for 300 different glycans in purified proteins, serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.

RayBio® Lectin Array 95. Detects glycan profiles using 95 lectins. Suitable for all liquid sample types.

RayBio® Lectin Array 70. Detects glycan profiles using 70 lectins. Suitable for all liquid sample types.

High-throughput live-cell analysis system

Multiplate live-cell analysis system

The Griess Reagent System measures nitrite (NO2–), which is one of two primary stable and nonvolatile breakdown products of nitric oxide (NO). Nitric oxide is an important physiological messenger and effector molecule in many biological systems, including immunological, neuronal and cardiovascular tissues. This assay relies on a diazotization reaction that was originally described by Griess in 1879. Through the years, many mod…

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase.   The luminescent signal positively correlates with kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary scre…

The Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamate in biological samples.

The Glutamine/Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples.

Count on minimal interference from test compounds when screening your compound library using luminescent, ATP-based kinase assays. We offer the ADP-Glo™ Kinase Assay, Kinase Selectivity Profiling Systems and hundreds of Kinase Enzyme Systems. These sensitive, reliable assays are easily scaled to meet your throughput needs and can be used with virtually any kinase and kinase substrate combination.

Measure Kinase-Ligand Affinity in Live Cells The NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay measures compound binding at select target kinases in intact cells. The assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET™ TE Assay measures the apparent affinity of test compounds by competitive displacement of the NanoBRET™ tra…

Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

ARRALYZE 8 Chamber Slide combines standard slide format with advanced nanowell technology, featuring 8 chambers with 127 precisely etched nanowells (0.4 × 0.4 × 0.225mm) per chamber. The borosilicate glass bottom (0.175μm thick under wells) provides superior optical clarity for high-resolution imaging while maintaining an inert environment. Includes 3 fiducials per chamber for easy navigation and precise cell tracking.

A comprehensive collection of active, recombinant protein kinases, crucial for screening and profiling small molecule inhibitors and studying cellular signaling pathways in disease.

These ready-to-use single positive biomarker controls are the latest and greatest from the Slingshot Team.

This kit is designed to measure the phosphotransferase activity in a kinase cascade reaction initiated by activated Raf-1. Recombinant unactive MEK1 is the substrate for Raf-1. Once activated by Raf-1, MEK1 can phosphorylate and thereby activate another kit component, recombinant unactive MAP Kinase 2/Erk2. Activation of MAP Kinase 2/Erk2 is determined by phosphorylation of myelin basic protein (MBP), in vitro. Phosphorylation…

The assay kit is designed to measure B-Raf dependent phosphotransferase activity in a kinase cascade reaction using recombinant MEK1, unactive as a B-Raf substrate. Recombinant MAPK2/Erk2, unactive is phosphorylated and activated by the activated MEK1 leading to phosphorylation of a MAP Kinase substrate, myelin basic protein (MBP). [γ-32P]ATP is the final phosphate donor.

Intended for the assay of recombinant B-Raf (supplied) kinase activity with experimental variables as determined by the user. For assay of B-Raf activity from a cell lysate, we recommend an immunoprecipitation-kinase assay, using anti-B-Raf antibody (07-453).

Intended for the assay of recombinant Raf-1 (supplied) kinase activity, with experimental variables as determined by the user. For assay of Raf-1 activity from a cell lysate, we recommend an immunoprecipitation-kinase assay, using anti-Raf-1 antibody (07-396).

A new secondary messenger for Gq coupled receptor investigation.Cisbio’s IP-One assay, based on its proprietary HTRF® technology, is the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger which tightly correlates with Gq-coupled receptor activity. This assay displays all the characteristic advantages of an HTRF® system: totally homogeneous, detection via fluorescence, signal stab…

This HTRF kit detects changes of cAMP accumulation in response to Gs coupled GPCR activation or inhibition.