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NanoBRET™ TE Intracellular Kinase Assays

Promega Corp.N2500, N2520, N2600,Available: Worldwide

Measure Kinase-Ligand Affinity in Live Cells The NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay measures compound binding at select target kinases in intact cells. The assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET™ TE Assay measures the apparent affinity of test compounds by competitive displacement of the NanoBRET™ tra…

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Description

Description

Measure Kinase-Ligand Affinity in Live Cells

The NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay measures compound binding at select target kinases in intact cells. The assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET™ TE Assay measures the apparent affinity of test compounds by competitive displacement of the NanoBRET™ tracer, reversibly bound to a NanoLuc^® luciferase-kinase fusions in cells.

How it Works
Using the NanoBRET™ TE Kinase Assay, a fixed concentration of tracer (K-4 or K-5) is added to cells expressing the desired NanoLuc^® kinase fusion to generate a BRET signal. Introduction of competing compounds results in a dose-dependent decrease in NanoBRET™ signal, which allows quantitation of the intracellular affinity of the target protein for the test compound.

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NanoBRET™ TE Intracellular Kinase Assays - Promega Corp. - Life Sciences

NanoBRET™ TE Intracellular Kinase Assays

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