Cell-Based Assays Products & Reviews

The DPPIV-Glo™ Protease Assay is a homogeneous, luminescent assay that measures dipeptidyl peptidase IV (DPPIV) activity. DPPIV is a serine protease that cleaves N-terminal dipeptides from polypeptides with L-proline or L-alanine at the penultimate position. The DPPIV-Glo™ Assay provides a proluminescent DPPIV substrate, Gly-Pro-aminoluciferin, in a buffer system optimized for DPPIV and luciferase activities. The addition of a…

The Griess Reagent System measures nitrite (NO2–), which is one of two primary stable and nonvolatile breakdown products of nitric oxide (NO). Nitric oxide is an important physiological messenger and effector molecule in many biological systems, including immunological, neuronal and cardiovascular tissues. This assay relies on a diazotization reaction that was originally described by Griess in 1879. Through the years, many mod…

The AttoPhos® AP Fluorescent Substrate System provides a highly sensitive fluorescent alkaline phosphatase substrate.   The system includes AttoPhos® Substrate, AttoPhos® Buffer and Calibration Solution. AttoPhos® Substrate (2′-[2-benzothiazoyl]-6′-hydroxybenzothiazole phosphate [BBTP]) is cleaved by alkaline phosphatase to produce inorganic phosphate (Pi) and the alcohol, 2′-[2-benzothiazoyl]-6´-hydroxybenzothiazole (BB…

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase.   The luminescent signal positively correlates with kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary scre…

NanoLuc® Binary Technology (NanoBiT) is a two-subunit system based on NanoLuc® luciferase that can be applied to the intracellular detection of protein:protein interactions (PPIs) in live cells. The NanoBiT® system is composed of two small subunits, Large BiT (LgBiT; 18kDa) and Small BiT (SmBiT; 11 amino acid peptide), that are expressed as fusions to target proteins of interest. The LgBiT and SmBiT subunits have been independ…

The Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamate in biological samples.

The Glutamine/Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples.

The Succinate-Glo™ JmjC Demethylase/Hydroxylase Assay is a bioluminescent assay for detecting the activity of JumonjiC (JmjC) histone demethylases and many Fe(II)/α-ketoglutarate-dependent dioxygenases that use α-ketoglutarate as substrate and release succinate as a product.

Count on minimal interference from test compounds when screening your compound library using luminescent, ATP-based kinase assays. We offer the ADP-Glo™ Kinase Assay, Kinase Selectivity Profiling Systems and hundreds of Kinase Enzyme Systems. These sensitive, reliable assays are easily scaled to meet your throughput needs and can be used with virtually any kinase and kinase substrate combination.

The NanoBRET™ Target Engagement (TE) Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. This target engagement assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells

Directly Measure HDAC-Test Compound Affinity and Residence Time in Live Cells

Measure Kinase-Ligand Affinity in Live Cells The NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay measures compound binding at select target kinases in intact cells. The assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET™ TE Assay measures the apparent affinity of test compounds by competitive displacement of the NanoBRET™ tra…

BRET-Based Assays to Detect Protein:Protein Interactions in Live Cells

Efficient Lysis of E. coli Cultures; No Centrifugation or Mechanical Disruption Needed FastBreak™ Cell Lysis Reagent is provided as a 10X concentrate and contains a proprietary nonionic detergent to facilitate lysis. Add the reagent directly to E. coli cultures. Following a brief incubation, the cells are disrupted, and the protein  released. Recombinant proteins can be directly screened in the cell extract or purified by t…

Single-Use Ligands for High-Resolution lmaging in Live Cells

Digitonin can be used in cell viability and cytotoxicity assays. It is a detergent solution useful for permeabilizing cells and for creating a cytotoxicity chemistry positive control. Supplied at 20mg/ml in DMSO

Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

COMET™ is a fully automated, high-throughput, hyperplex platform for the analysis of Formalin-Fixed, Paraffin-Embedded (FFPE), and frozen tissues.

Tools to acelerate biomarker identification and validation

Lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified

Developing a precise, accurate, and reliable assay is never easy. From raw material sourcing, to technical considerations, to optimization and ultimate scale-up, a development team counters challenges that strain resources and delay timetables. Savvy developers recognize that assay development and optimization move smoother when optimal raw materials and expert support are identified early in the process.

Nanion's Orbit 16 TC platform takes the pain out of bilayer painting and subsequent recordings as it enables fast and successful data generation thanks to the rapid automated formation of 16 bilayers at once and their subsequent fully parallel recording.

The SURFE²R N1 is an automated analysis device for the measurements of electrogenic transporters (symporters, exchangers and uniporters) and pumps. It employs the label-free, SSM-based electrophysiology technology, achieving a throughput of 150 datapoints per day.

PAX-8 (MRQ-50) Mouse Monoclonal Antibody