Antibody Humanization Service
Sino BiologicalSino Biological offers top-notch monoclonal antibody humanization services utilizing complementarity-determining region (CDR) grafting technology and computer-aided molecular modeling.
Antibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.
Sino Biological offers top-notch monoclonal antibody humanization services utilizing complementarity-determining region (CDR) grafting technology and computer-aided molecular modeling.
The ChIPAb+ Acetyl-Histone H3 set contains purified rabbit polyclonal antibody and the normal rabbit IgG antibody, which can be used to demonstrate that the acetyl-histone H3 antibody is capable of precipitating acetyl-histone H3 associated chromatin. The qPCR primers included flank the human GAPDH promoter and produce a 166 base pair PCR product.
The ChIPAb+ Trimethyl-Histone H3 (Lys9) set includes the Anti-trimethyl-Histone H3 (Lys9) antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 170 bp region within the promoter of the murine p16 gene. The trimethyl-histone H3 (Lys9) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of trimethyl-…
The His•Tag® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of Novagen's expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10 to 8–1 × 10–9 M) enables sensitive, specific detection of His•Tag fusion prote…
Product Type: antibody. Immunogen: native, from brain. Host: mouse. Type: monoclonal. Clone: 31A3. Subclass: IgG1. Format: affinity purified Ig. Preparation: prepared by Protein A chromatography. State: liquid. Buffer: PBS buffer, pH 7.2, 0.1% Sodium Azide. Storage: aliquot and store at -20°C.Cross reactivity Reacts with PGP 9.5 from human and rat. Tissue reactivity Stains neuronal cell bodies and axons in the CNS and peripher…
Product Type: antibody. Immunogen: native, from plasma. Host: mouse. Type: monoclonal. Clone: 10-11. Subclass: IgG1-k. Format: affinity purified Ig. Preparation: Protein A chromatography. State: liquid. Buffer: borate buffered saline, pH 8.4, 0.1% sodium azide. Storage: aliquot and store at -20°C.Cross reactivityDoes not react with C4c. Does not cross react with cow, cat, dog, guinea pig, mouse, rabbit, sheep or rat.Specificit…
Product Type: antibody. Immunogen: native. Host: goat. Type: polyclonal. Format: purified Ig. Preparation: Ammonium Sulphate fractionation. State: liquid. Buffer: PBS buffer, pH 7.2, 0.1% Sodium Azide. Storage: aliquot and store at -20°C.ImmunohistochemistryThis antibody, when tested in the serum format, has given variable results in IHC. The purified product has not been evaluated for this application by Biogenesis.ELISAIn a…
Product Type: antibody. Immunogen: native, purified. Host: sheep. Type: polyclonal. Format: serum. Preparation: neat. State: liquid. Buffer: without preservatives. Storage: aliquot and store at -20°C.RIA 1/4K - 1/20K (125 I) IHC to be determined Cross reactivity rat GH 100% rat ACTH Suggested protocol Overnight (16-24 hr) equilibrium assay with DAB/PEG separation. 100 µl standards, 100 µl tracer (18,000 cpm) and 200 µl primary…
Product Type: antibody. Immunogen: native. Host: mouse. Type: monoclonal. Clone: 5B6/6. Subclass: IgG1-k. Format: affinity purified Ig. Preparation: Protein A chromatography. State: liquid. Buffer: PBS buffer, pH 7.4, 0.1% Sodium Azide. Storage: aliquot and store at -20°C.Cross reactivityReacts with human, porcine, bovine, rat and mouse insulin and proinsulin.SpecificityEpitope recognised is different from that identified by 5…
Product Type: antibody. Immunogen: BSA conjugate. Host: mouse. Type: monoclonal. Clone: V/h1. Subclass: IgG1. Format: affinity purified Ig. Preparation: Protein A chromatography. State: liquid. Buffer: PBS buffer, pH 7.2, 0.1% Sodium Azide. Storage: aliquot and store at -20°C.RIA titre to be determined ELISA titre to be determined IHC titre to be determinedCross-reactivityReacts with pure MBP from sheep, rabbit, guinea-pig and…
Product Type: antibody. Immunogen: native, see notes. Host: mouse. Type: monoclonal. Clone: AE5. Subclass: IgG1. Format: affinity purified Ig. Preparation: Ion Exchange chromatography. State: liquid. Buffer: PBS buffer, pH 7.2, 0.1% Sodium Azide. Storage: aliquot and store at -20°C.Cross reactivityReacts with bovine and rabbit snout and lip epithelium. Reacts with some oral mucosa epithelium. This antibody also reacts with rab…
Product Type: antibody. Immunogen: native, from umbilical cord. Host: sheep. Type: polyclonal. Format: affinity purified Ig. Preparation: prepared by Protein G chromatography. State: liquid. Buffer: PBS buffer, pH 7.2, 0.1% Sodium Azide. Storage: aliquot and store at -20°C.ELISA 1/50 - 1/400
Product Type: antibody. Immunogen: native, from placenta. Host: rabbit. Type: polyclonal. Format: affinity isolated. Preparation: affinity chromatography. State: lyophilised. Buffer: contains mannitol, dextran and salts. Storage: store at -20ºC, aliquot reconstituted at -20ºC.Cross reactivityHuman collagen type III SpecificityThis antibody reacts with native and heat denatured collagen type I. Human plasma proteins do not inte…
Quick and Easy Antibody Quantitation:With its simplicity and ease of use, Guava’s new RapidQuantTM IgG Kits have raised the bar for IgG titering assays. Designed to be time-saving, labor-saving and money-saving, Guava’s RapidQuant kits eliminate the most tedious, laborious portions of antibody titering methods with Guava’s signature ease of use. • Robust, rapid and highly reproducible • Relevant quantitative range • Quantifies…
Diagenode has revolutionized immunoprecipitation with a novel, rigorous antibody validation system. We have partnered with epigenetics experts including the BLUEPRINT Epigenome consortium to develop the absolute best epigenetics antibodies with the highest specificity and sensitivity available. These Diagenode Validated Antibodies are categorized into three different classes (Premium, Classic, and Pioneer) dependent on their…
With MedixMAB by Design recombinant antibodies you can create next-generation assays: Develop superior assays with high sensitivity or specificity Generate antibodies against difficult antigens with low immunogenicity or toxic compounds Improve existing antibodies by: Improving the affinity Reducing cross-reactivity Increasing efficiency Enables flexible and fast production
A breakthrough in genome engineering. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate up- or downregulation of specific endogenous genes or to alter hi…
Afla-V AQUA Strip Tests utilize the proven sensitivity and selectivity of VICAM's monoclonal antibodies to accurately detect and measure total aflatoxins (B1, B2, G1 & G2) at levels as low as 2.5 ppb and as high as 50 ppb. Afla-V AQUA eliminates the need for hazardous solvents using a water-based, dilution-free extraction procedure that develops in just 5 minutes. Results are displayed on the digital screen and may also be pr…
Send us your antibody and let our ChIP Experts™ perform validation of your antibody for ChIP applications
Reduce labor costs by using our ready-to-use Lateral Flow Coupling Kit for magnetic and latex particles. No messy pre-preparation of reagents required. Simply pipette Anteo's water-based Activation Reagent onto your chosen particles to activate. Hands-on time for surface activation and coupling is 60 minutes. Magnetic particles are easier to use than gold. Magnetic separation removes the need to perform centrifugation and f…
Anteo’s ready-to-use Antibody Coupling Kit for magnetic, polystyrene and silica particles is a less hazardous alternative to covalent NHS/EDC chemistry. No messy pre-preparation of reagents required. Simply pipette Anteo's water-based Activation Reagent onto your chosen particles to activate. Hands-on time for surface activation and coupling is 60 minutes. With this kit, antibodies assemble in the correct…
Reduce labor costs by using our ready-to-use Magnetic Separation Coupling Kit. No messy pre-preparation of reagents required. Simply pipette Anteo's water-based Activation Reagent onto your chosen particle to activate. Hands-on time for surface activation and coupling is 60 minutes. This kit greatly reduces the risk of antibody damage, leading to less antibodies required for the experiment, increased antibody functionality,…
An extensive range of antibodies from BioPorto and Wako suitable for use in various immunoassay techniques including: ELISA, Western Blotting, Immunohistochemistry and Immunoprecipitation. Products Include: Variability Free IgE Immunohistochemistry stains and reagents Recombinant Proteins Primary Antibodies Secondary Antibodies
The StarBright Blue 700 Fluorophore is an ultra-sensitive polymer-based fluorophore with an excitation maximum at 470 nm and emission maximum at 705 nm. StarBright Blue 700 is a brighter alternative to PerCP-Cy5.5, with less spillover, and does not exhibit tandem breakage.
Excellent marker for wild-type and mutant forms of human p53 antigen