StarBright™ Blue 520 Fluorescent Secondary Antibodies

StarBright Blue 520 Conjugated Goat Anti-Mouse and Goat Anti-Rabbit Fluorescent Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single-target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. StarBright Blue 520 Fluorescent Secondary Antibodies can be used with far-red emission StarBright Blue 700 Antibodies as well as traditional fluorescent detection reagents like Alexa Fluor and DyLight Conjugated Antibodies. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or with hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.

Features and Benefits

• Easy multiplexing — can be used with traditional fluorescent antibodies or with Bio-Rad's StarBright Blue 700 Fluorescent Secondary Antibodies to detect multiple proteins on the same blot
• High sensitivity — StarBright Blue 520 Fluorescent Secondary Antibodies (Exmax/Emmax = 488 nm/520 nm) have 2–4 fold higher sensitivity compared to traditional green emitters like Alexa Fluor 488 or DyLight 488 Antibodies
• Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes
• Low nonspecific binding — the StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies

Applications and Uses

StarBright Blue 520 Fluorescent Secondary Antibodies can be used for multiplex fluorescent western blotting with Bio-Rad's ultra-sensitive near IR StarBright Blue 700 Antibodies and the hFAB Rhodamine Anti-Housekeeping Antibodies as described in the table below.