Products & Reviews

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution.   The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA. The…

The GoTaq® G2 Hot Start Polymerase and Master Mixes simplify your reaction preparation, bringing you the convenience of room-temperature setup and the specificity of a hot start polymerase.   Ready-to-use Master Mix simplifies reaction setup and allows direct-to-gel loading. The optimized formulation provides amplification of a variety of targets, while eliminating non-specific products, resulting in higher yields and gr…

The AttoPhos® AP Fluorescent Substrate System provides a highly sensitive fluorescent alkaline phosphatase substrate.   The system includes AttoPhos® Substrate, AttoPhos® Buffer and Calibration Solution. AttoPhos® Substrate (2′-[2-benzothiazoyl]-6′-hydroxybenzothiazole phosphate [BBTP]) is cleaved by alkaline phosphatase to produce inorganic phosphate (Pi) and the alcohol, 2′-[2-benzothiazoyl]-6´-hydroxybenzothiazole (BB…

Polyclonal secondary antibodies raised in goat, rabbit or donkey, immunoaffinity-purified using corresponding immobilized antigens and conjugated to horseradish peroxidase (HRP) enzyme.   The Anti-Human IgG (H+L), HRP Conjugate, Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate antibodies bind to both heavy and light chains for all IgG subclasses. As with all antibodies, in certain applications…

The MagneHis™ Protein Purification System provides a simple, rapid and reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins.   Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Using a tube format,…

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues.   The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in addition…

The Kazusa ORFeome Project was initiated to create a collection of human and mouse ORFs to enable the larger research community with a readily accessible, highly validated ORFeome. Kazusa DNA Research Institute (KDRI) is an academic Japanese research institute dedicated to genome-scale studies and the function of individual genes. KDRI is an active member of the global ORFeome Collaboration, and are experts in HUGE (Human Uni…

The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression.   These cells consolidate the best attributes of these two steps into one strain to evaluate protein expression in E. coli. Transformation efficiencies are greater than 108 cfu/μg, similar to other highly competent cells. The single step cells are available in single transformation size (50μl). KRX a…

For standard cloning experiments competent cells are available in two formats: high efficiency at greater than 108cfu/μg for cloning (JM109, HB101), and subcloning efficiency at greater than 107cfu/μg (HB101). JM109 cells have the additional feature of blue/white screening for recombinants. For protein expression, JM109(DE3), BL21(DE3) or KRX competent cells can be used. The Single Step (KRX) Competent Cells are designed fo…

Recombinant RNasin® Ribonuclease Inhibitor reliably protects your RNA samples from RNase degradation.   RNases are ubiquitous and degradation can cause significant damage to RNA, hindering downstream reactions. Protect your sample with the most robust inhibitor available-- Recombinant RNasin® Ribonuclease Inhibitor. Recombinant RNasin Ribonuclease Inhibitor Features & Benefits:   Inhibits a broad spectrum o…

Diamond™ Nucleic Acid Dye is a safe, sensitive fluorescent dye that binds to single-stranded DNA, double-stranded DNA and RNA, and can be used to stain and visualize nucleic acids in gels.   Diamond™ Nucleic Acid Dye is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system. Significantly less mutagenic than traditional ethidium bromide, as proven by Ames…

The BenchTop DNA Markers, supplied in a stabilizing solution of 1X Blue/Orange Loading Dye, come in a variety of different sizes to meet your DNA electrophoresis needs.

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes).   Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification pri…

The PureYield™ Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA.   The system provides a rapid method for purification of up to 15μg of plasmid DNA from 600μl to 3ml of bacteria culture. Plasmid DNA can be purified in as little as 10 minutes. The PureYield™ Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxi…

Promega has partnered with ATCC to offer unparalleled STR testing service for human cell line authentication.   This service provides easy access to proven and comprehensive validation techniques. Promega's proprietary PowerPlex® and GenePrint® STR technology coupled with ATCC's considerable experience profiling human cell lines and extensive internal assets around STR profiling data interpretation is particularly r…

High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling.   Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dNTPs Features & Benefits:   Dependable - PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzyme…

The PowerPlex® 21 System is a multiplex STR system for human identification applications including forensic analysis, relationship testing and research use.   The PowerPlex® 21 System is more tolerant of PCR inhibitors than other STR systems including the PowerPlex® 16 HS System. This allows more interpretable data and less need for re-amplification of samples previously deemed "difficult" due to the presence of inhibito…

The PowerPlex® ESX 17 and ESI 17 Fast Systems meet recommendations of the European Network of Forensic Science Institutes (ENFSI) for DNA profile sharing across Europe. They are based on five-color technology and allow co-amplification and detection of D3S1358, D8S1179, D18S51, D21S11, FGA, TH01, vWA, D2S441, D10S1248, D22S1045, D1S1656, D12S391, D2S1338, D16S539, D19S433, SE33 and Amelogenin. These kits are available in…

GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polyemrase in a specially formulated mixture with a proprietary thermostable proofreading polyemrase.   This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The presence of a proofreading enzyme to repair DNA mismatches and a highly processive polyemrase allows the polyemras…

QuantiFluor® ONE system maximizes sensitivity and efficiency for dsDNA quantitation.   The QuantiFluor® ONE dsDNA System contains a fluorescent double-stranded DNA-binding dye (504nmEx/531nmEm) developed for use in an “add-and-read” format for dye and standard, making sample quantitation easy. This system enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA). The QuantiFluor® ONE dsDNA Sy…

The Quantus™ Fluorometer is an easy-to-use and affordable single-tube fluorometer.   The highly sensitive instrument is preprogrammed with QuantiFluor® Dye protocols for single-stranded DNA, double-stranded DNA and RNA quantitation but is also compatible with other fluorescent assays. Quantus™ Fluorometer does the math for you and displays your concentration after each measurement automatically. No computer is…

This ready-to-use multimode detection system includes: high-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities.   The GloMax® Discover System is a high-performance multimode detection instrument developed with Promega reagent chemistries to provide a simple means of detecting advanced chemistries. This instrument provides…

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase.   The luminescent signal positively correlates with kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary scre…

The Maxwell® Clinical Sample Concentrator (CSC) automated nucleic acid extraction system is designed for clinical laboratories needing to process multiple samples for critical downstream assays. This easy-to-use, cGMP-compliant system provides consistent, high-quality results with every run, allowing you to manage your workflow and gain efficiency. The purification methods use sample lysis and binding to paramagnetic par…

Designed for use with the Maxwell Clinical Sample Concentrator and the Maxwell CSC blood purification method as an IVD medical device for automated isolation of human genomic DNA from whole blood.   Because the Maxwell® CSC Blood DNA Kit is manufactured to such strict specifications, you can count on consistent, high quality DNA suitable for use in downstream amplification assays. Furthermore the intuitive interface…