PCR and Thermal Cycling Products & Reviews

High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling.   Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dNTPs Features & Benefits:   Dependable - PCR-tested deoxynucleotides ensure optimal performance with all Promega amplification enzyme…

GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polyemrase in a specially formulated mixture with a proprietary thermostable proofreading polyemrase.   This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The presence of a proofreading enzyme to repair DNA mismatches and a highly processive polyemrase allows the polyemras…

Preformulated PCR Master Mix Components to Determine Optimal PCR Amplification Conditions

Sensitive Quantitation of Single-Stranded DNARequires less DNA than spectrophotometry

Inhibits Polymerase Activity at Temperatures below 70°C High conc and glycerol-free formulations

Inhibits Polymerase Activity at Temperatures below 70°C High conc and glycerol-free formulations

Inhibits Polymerase Activity at Temperatures below 70°C High conc and glycerol-free formulations

Confidence for Mixed Sample Analysis Track and confirm tissue specimen provenance and cell lineage

cGMP-Grade Solution of dNTPs Pretested in PCR- Two concentrations available: 10mM and 25mM

Process swabs and storage card punches for direct amplification.

Quantify autosomal and male (Y) DNA, assess the level of DNA degradation and detect PCR inhibitors.

Get robust, reliable performance with GoTaq® G2 Hot Start Polymerase

Full-Length cDNA Synthesis for the Analysis of Rare or Long Messages

Optimized PCR master mix: Just add template, primers and water

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading ac…

GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA inp…

GoTaq® qPCR Master Mix is a new reagent system for use in real-time quantitative PCR (qPCR). The system contains BRYT™ Green, a new proprietary fluorescent DNA binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA than SYBR® Green I. Combined with the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, GoTaq® qPCR Master Mix provides robust real-time PCR with increased re…

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The system is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so tha…

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibit…

Modifications that enhance the performance of oligonucleotides

Product features Pink or light blue above 7°C, violet or dark blue when ice-cold DescriptionThe new Eppendorf PCR-Cooler maintains a sample temperature of 0°C*1 for over an hour and then changes color when the temperature has exceeded 7°C. Superior to an ice bath, PCR-Cooler is easy to handle, space-saving and free of contamination, which makes it the perfect preparation for your PCR reactions. The temperature of single tub…

Eppendorf Mastercycler is the economical option when, after having optimized your PCR using Mastercycler gradient, you wish to carry out an experiment with the usual Mastercycler quality, but without blocking your optimization cycler.The entire Eppendorf Mastercycler family boasts the personal card system. Chip-based memory cards enable not only simple and reliable transfer of your individual protocols between different Master…

The Eppendorf CycleManager is a Windows based software for programming and controlling one or more Mastercyclers. The thourough databank ensures reliable storage of all GLP relevant experimental data incl. detailed user comments.The main dialogue box is the central user interface. All key functions can be accessed from it. Mastercyclers can be added or removed from the Multi-Cycler-Network. It offers also an overview of all co…

• Strips with 8 microcaps for 0.2 ml wells• Easy and rapid sealing of Eppendorf twin.tec plates and other standard microtiter plates, see also Capping Aid• Easy to remove using extension at the end of the strip• Autoclavable (121°C, 20 min.)• Certified free of human DNA, DNase, RNase and PCR Inhibitor**Certificate, test procedures and detailed information on request.

Rack for handling and storing tubes and plates For 0.2 ml, 0.3 ml, 0.5 ml tubes and 96-well microtiter plates Stackable Can be stored down to -90°C Autoclavable (121°C, 20 min.)