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Hygiena's new BAX System Real-Time PCR Assay E. coli precisely detects deadly bacteria in food

Hygiena

Improved assay reduces time to product release by detecting bacteria faster

6 Nov 2020
Diane Li
Assistant Editor

Product news

Hygiena, a leader in food safety testing, has announced the NEW BAX® System Real-Time PCR Assay for E. coli O157:H7 Exact, a test that can specifically identify Escherichia coli O157:H7 bacteria (and not similar serotypes) in less time than other tests.

The BAX® technology is based on the polymerase chain reaction (PCR) method of amplifying minute amounts of genetic material in a sample for more accurate measurement. The new BAX® System Real-Time Assay for E. coli O157:H7 Exact is based on a single genetic target in E. coli O157:H7, providing a definitive result for immediate action and minimizes the need for additional confirmatory testing.

Improved chemistry in the new assay allows detection as low as 104 colony forming units (CFU/mL) in an enriched sample, opening the door to reduced enrichment times and faster time to results for quicker product release.

The assay can be run simultaneously with the BAX® System Real-Time PCR assay suite for STEC, including the BAX® System Real-Time STEC screening assay, and BAX® System Real-Time PCR assays for STEC Panel 1 and STEC Panel 2.

Hygiena’s validation testing of the BAX® System Real-Time PCR Assay for E. coli O157:H7 Exact in raw ground beef, beef trim, leafy greens and raw milk showed no significant differences in results compared to the reference conventional culture confirmation method. In addition, the test successfully identified 239 strains of E. coli O157:H7 and did not identify 300 non-O157:H7 strains or 175 non-E. coli bacteria.

“We are so excited to introduce this new assay to the BAX® System! This assay will dramatically improve the accuracy and speed of testing for E. coli O157:H7, providing the industry a needed tool for product release,” said April Englishbey, PhD., product manager at Hygiena. “We are confident that this assay’s ability for improved results in a variety of tough matrices will help improve the safety of food supply chains.”

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.Food and Beverage AnalyzersFood and Beverage Analyzers are commonly used to provide information about the chemical composition, structure and physical properties of food materials.Food and Beverage ProcessingFood and Beverage Processing involves the preparation of food products for further analysis. Typical instrumentation includes blanchers, color sorters, cookers, grinders, and refrigeration. These are used to replicate the processes commonly used in the commercial preparation of food and beverages.Particle CharacterizationParticle characterization instruments are used to determine particle size distribution, shape, surface area, zeta potential, density and porosity of particles and materials. Multiple tecchniques are available for determining particle size, shape and count including dynamic light scattering (DLS), laser diffraction, electrozone (Coulter technique), imaging particle analysis and single particle optical sensing. Determine the density of your material with a gas pycnometer or examine its surface area and porosity with gas adsorption analyzers and mercury porosimeters. Find the best particle characterization instruments in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Escherichia coliFood SafetyFood safety describes the prevention of food-borne illnesses. Contamination with a variety of pathogens can be prevented with adequate temperature control, hygiene and labeling. 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Advanced testing methods like PCR, chromatography, and spectroscopy are used to identify contaminants in food products. Browse our peer-reviewed product directory to find the best solutions for food contamination detection, compare products, check reviews, and get pricing directly from manufacturers.Assay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.Food QualityFood quality refers to the attributes of food that influence its safety, nutrition, and taste. Monitoring and ensuring food quality are essential in production to meet health regulations and consumer expectations. Techniques like spectroscopy, chromatography, and microbial testing are used to assess food properties and detect contaminants. Browse our peer-reviewed product directory to find food quality testing solutions; compare products, check reviews, and get pricing directly from manufacturers.Chemical Residues in Food

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Hygiena's new BAX System Real-Time PCR Assay E. coli precisely detects deadly bacteria in food