Comparing fluorescence and Trypan Blue automated cell counting
20 Dec 2021
Trypan Blue is widely used in cell culture labs for selectively staining dead cells and tissues. The use of Trypan Blue dye can present challenges for accurately assessing cell counts and viability in some sample types. For these samples, a fluorescence assay such as Acridine Orange / Propidium Iodide (AO/PI) is a simple and highly accurate alternative.
Using the CellDrop Automated Cell Counter, DeNovix Scientific Director Dan Schieffer demonstrates both brightfield and fluorescence methods of cell counting and viability assessment.
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DeNovix
DeNovix is a private company formed in 2011. As scientists and engineers with proven track records of commercializing innovative products, we are passionate about facilitating the advances being made in today’s scientific community. We live in an incredible time where the pace of research is outstripping the capabilities of modern instrumentation. We work with and for the primary research community to ensure new ideas are never limited by current technology.
Our mission is to develop, manufacture and sell innovative products for the life science community with an emphasis on higher technology optics and detection instruments. Our philosophy is to provide products of outstanding value to our customers.
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CellDrop™ Automated Cell Counters
DeNovixReduce consumable costs and improve lab sustainability with patented DirectPipette™ technology, or use slide mode for ultimate measurement flexibility. The CellDrop features dual fluorescence and brightfield optics, variable height sample chamber to make cell counting fast, simple, and reproducible.
