CellDrop™ Automated Cell Counters

Trypan Blue is widely used in cell culture labs for selectively staining dead cells and tissues. However, some sample types can present challenges for accurately assessing cell counts and viability with Trypan Blue. For these sample types, a fluorescence assay such as Acridine Orange / Propidium Iodide (AO/PI) is a simple and highly accurate alternative. Learn about each viability method and the situations where fluorescence is advantageous in this free eBook.

Isolating clean, intact nuclei is vital for the success of single-nucleus workflows such as RNA sequencing (RNA-seq) and Assay for Transposase-Accessible Chromatin with sequencing (ATAC-seq). High-quality, debris-free samples are essential for accurate library preparation and downstream analyses, as debris can hinder precise nuclei counting. Traditional manual counting methods, while reliable, are time-consuming and prone to human error, especially with large sample volumes.

This eBook explores automated nuclei counting and quality control using the DeNovix CellDrop™ Automated Cell Counter. With features like DirectPipette™ technology that eliminates costly consumables, advanced fluorescence applications, and user-friendly software, researchers can improve accuracy, efficiency, and consistency in sample preparation. Discover practical tips to streamline workflows, minimize variability, and enhance experimental outcomes.

The requirement for ever-greater focus and precision has driven the development of improved single-cell sequencing analysis technologies. Techniques such as RNA sequencing and ATAC sequencing require intact single nuclei, so the ability to isolate individual nuclei from sample tissue is therefore critical to the success of these sequencing approaches. Isolating nuclei that are non-clustered and free from debris is critical both to successful library preparation and the ability to count nuclei accurately.

To help you achieve isolation of nuclei for your single-cell genomics workflows, this expert guide outlines:

• Automated counting of isolated nuclei
• Best practices for cell counting
• Improving cell counting accuracy

It will also cover two case studies – frozen brain tissue and plant protoplasts – including technical notes.

Download your free copy to learn more about the key features and applications of the CellDrop™ FL Automated Cell Counter from DeNovix as well as how the DeNovix DirectPipette™ technology is designed to eliminate plastic slides and cumbersome hemocytometers from routine cell counting.

In this application note, DeNovix introduces the CellDrop™ automated cell counter, which offers cell counting without the use of plastic slides. The CellDrop features dual fluorescence and brightfield optics, a variable height sample chamber, and powerful analysis software. It can quantify and qualify the viability of primary cell samples, such as peripheral blood mononuclear cells (PBMCs) using viability dyes to count whole blood samples. It is also optimized for accurate cell counts and viability across a wide range of sample types, including those with significant debris or minimal debris. Recommended viability assays are provided for common sample types.

Take the hassle out of preparing sample slides for cell counting with the CellDrop™ Automated Cell Counter. This user-friendly device is designed to load cell suspensions directly onto durable, scratch-proof sapphire surfaces, eliminating the need for disposable slides. Watch in this video demonstration as it delivers a live HD view of cells and produces an accurate brightfield cell count in less than three seconds.

Discover how the CellDrop™ automated cell counter overcomes the challenges of counting organoids, tumorspheres, and spheroids. See how advanced machine learning enhances accuracy, and watch a live demo of organoid counting in action.

In this on-demand webinar, DeNovix Application Scientist Ben Capozzolli discusses a method for accurate and reproducible nuclei counts and shares tips for preparing nuceli samples before single-cell sequencing workflows.

In this video, DeNovix application scientist, Ben Capozzoli, demonstrates the process of counting single nuclei suspensions on the CellDrop™ Automated Cell Counter. He emphasizes the significance of isolating nuclei for various workflows like ATAC-sequencing and RNA-sequencing. Having precise nuclei counts and knowledge of debris content is crucial for successful downstream applications. The CellDrop excels in accurately determining nuclei counts and extraction efficiency scores, irrespective of debris presence.

The new software is offered as a free upgrade for existing customers

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DeNovix has released a new model in the CellDrop Automated Cell Counter range, with enhanced performance and new cell counting applications

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