Product DemonstrationsLife Sciences

Celloger Mini Plus application: Nocodazole-mediated mitotic arrest

28 Jun 2023
Celloger Mini Plus application: Nocodazole-mediated mitotic arrest

In this video, CURIOSIS examines the anti-mitotic activity of nocodazole against a cancer cell line by monitoring cell division. HeLa cells, transfected with H2B-GFP plasmid, are seeded in a 24-well plate at 4 x 104 cells per well. After allowing the cells to attach overnight, they are incubated with or without 62.5 nM of nocodazole. Real-time imaging is conducted using the Celloger® Mini Plus 4x objective, capturing green fluorescence. Time-lapse images are taken every 15 minutes over a period of 19.5 hours.

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CURIOSIS

“Curiosity is the most powerful assets you own”

Curiosis is an innovative biotech company to provide integrated solutions in the field of Life science, especially in cell-based application. Curiosis’ expert team of scientists and engineers is dedicated to developing high-efficiency instruments by using core technologies of biophysics and electronic engineering. Providing cutting edge technologies and optimized platform allow the researchers to challenge their progress improvement.

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Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.