Cell line engineering with CRISPR-Cas9: Guide RNA & donors for knock-in
20 Oct 2020
Generating a knock-in cell line adds another line of complexity to the relatively straightforward knockout application. Guide RNA can be more tricky to design as you not only need more efficient guides, but they also need to be in close proximity to your insert site of interest. John Schiel, Ph.D., knock-in expert at Horizon Discovery shares how Horizon's tools for site specific editing and donor design aid in generating the sequences for these reagents as well as what donor formats make sense based on your type of insert.
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Revvity Signals
Revvity Signals offers one of the most comprehensive suites of scientific software in the world. Its powerful informatics solutions are used across a spectrum of industries including Pharma and Biotech, Specialty & Agro-Chemicals, Energy & Petrochemicals, Flavors & Fragrances, Food & Beverage and Electronics. From its internationally recognized flagship ChemDraw® and E-Notebook applications, to its Signals Research Suite (Signals Notebook, Signals VitroVivo and Signals Inventa) to its exclusive TIBCO® Spotfire® partnership that brings scientific data analytics to visual life, no scientific company offers a wider range and more powerful suite of scientific solutions than Revvity Signals.
