Affinity measurements with the Monolith X
4 Jun 2024
Application Scientist Nathan Wallace, PhD, introduces the Monolith X instrument, emphasizing its features like easy assay development and quick KD measurement. It discusses the instrument's versatility in testing different molecules and buffers. The two biophysical modalities, spectral shift technology and MST, are explained. The process of sample preparation, software usage, and assay optimization is also covered.
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Monolith X for Immobilization-Free Binding Affinity Measurements
NanoTemper TechnologiesMonolith X is a capillary-based system that enables you to measure the strength of molecular interactions in solution, without the need for immobilization. Minimal impact fluorescent labelling empowers you to measure Kds between a wide variety of targets and ligands using low amounts of sample. Enjoy approachable assay development with a fully guided process embedded in the default software.
