ResourceDrug Discovery & Development
The power of in-line mixing for competitive compound analysis
24 Oct 2025This application note introduces NeXtStep™ injections on the Octet® SF3 SPR system, a powerful method for performing rapid, in-line mixing competition assays without the need for pre-mixed samples. Learn how this technique simplifies assay setup, reduces false positives, and enables accurate site-specific binding analysis. Featuring real-world examples with sulfonamides, the note demonstrates how NeXtStep™ enhances fragment screening and small molecule characterization with high sensitivity and efficiency.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Biophysical CharacterizationBinding Kinetics