Sorting a 12-color immunophenotyping pannel

6 Oct 2019

In this application note, the MA900 cell sorter was used for analysis and sorting of a 12-color immunophenotyping panel. Human peripheral blood mononuclear cells (PBMCs) were stained with reagents for analysis of common T-cell, B-cell, NKcell, and monocyte subsets. These subsets were identified using different gating strategies, and sort performance was evaluated by performing 4-way sorting of T cells, NK cells, B cells, and monocytes, followed by post-sort analysis.

MA900 Multi-Application Cell Sorter

Sony Biotechnology

The MA900 from Sony meets the needs of most sorting applications, supporting 12 fluorescence parameters and 4-way sorting. Powerful, modern technologies built into the MA900 system include a patented micro fluidic chip-based design, comprehensive fluidic controls, and advanced automation that dramatically simplifies operation to make sorting less subjective and improve reliability.

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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.Cell SortingCell sorting isolates specific cell populations based on physical or molecular characteristics, essential in immunology, cancer research, and regenerative medicine. Techniques like flow cytometry improve accuracy and speed. Compare cell sorting instruments and solutions in our product directory.ImmunophenotypingBlood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.
Sorting a 12-color immunophenotyping pannel