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Real-time human T Cell activation with Agilent Seahorse XF Hu T Cell Activation Assay kit

Agilent Technologies
31 Jul 2023

In this application note, Agilent Technologies introduces the Agilent Seahorse XF Hu T Cell Activation Assay, extending XF technology for studying T cell activation. The assay offers rapid, standardized measurement of early activation-associated metabolic responses in human T cells. It provides insights into activation dynamics and metabolic changes, supporting immune cell therapies and drug discovery. The assay includes two experimental designs: a standard assay for testing T cell activation and a modulation assay for compound effects on activation. Examples demonstrate its application in T cell immunology and metabolism.

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Seahorse XF Hu T Cell Activation Assay Kit

Agilent Technologies

Agilent Seahorse XF Hu T Cell Activation Assay Kit measures human (Hu) T cell activation response within several minutes of stimulation using Seahorse XF Analyzers. This is accomplished via measuring proton efflux rates (PER) associated with glycolytic pathway-dependent energy production, and is built on the principle that T cell activation is accompanied by a rapid switch in cellular energy production and intermediate demand to support effective proliferation, size expansion, and differentiati

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Real-time human T Cell activation with Agilent Seahorse XF Hu T Cell Activation Assay kit