Seahorse XF Hu T Cell Activation Assay Kit
Agilent Seahorse XF Hu T Cell Activation Assay Kit measures human (Hu) T cell activation response within several minutes of stimulation using Seahorse XF Analyzers. This is accomplished via measuring proton efflux rates (PER) associated with glycolytic pathway-dependent energy production, and is built on the principle that T cell activation is accompanied by a rapid switch in cellular energy production and intermediate demand…
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The kit contains Agilent-validated ImmunoCult Human CD3/CD28 T Cell Activator and 2-deoxy-D-glucose (2-DG) and is an optimal solution delivered by Agilent and STEMCELL Technologies partnership for interrogating and modulating T cell activation on the Seahorse XF platform. The ImmunoCult Human CD3/CD28 T Cell Activator from STEMCELL Technologies is a soluble tetrameric complex. It is easy-to-use and compatible with specially designed injection ports on Agilent Seahorse XF cartridges, reducing assay preparation time and improving assay reliability. Together with standardized protocols and cloud-based Agilent Seahorse Analytics software, the XF Hu T Cell Activation Assay Kit provides a robust measure of T cell activation with new insights.
Features
- Detects human T cell activation in minutes, providing an early window to investigate cellular and signaling requirements associated with T cell activation
- Provides kinetic readout for continuous monitoring of human T cell activation and modulation
- Allows dosing of cells with test compounds/modulators during assay through the built-in injection ports
- The soluble ImmunoCult Human CD3/CD28 T Cell Activator (STEMCELL Technologies) is easy-to-use and compatible with specially-designed injection ports on Agilent Seahorse XF cartridges, reducing assay preparation time and improving assay reliability
- Reagents are directly reconstituted or diluted in the assay medium, eliminating the need for DMSO or other solvents
- Every lot is tested with functional assays to deliver consistent and robust results
- Upload your assay result file to your Agilent Seahorse Analytics account to calculate and report parameters of the XF T Cell Activation assay, share with collaborators, or export data to Microsoft Excel or GraphPad Prism for other graphing or analysis needs
Real-time human T Cell activation with Agilent Seahorse XF Hu T Cell Activation Assay kit
In this application note, Agilent Technologies introduces the Agilent Seahorse XF Hu T Cell Activation Assay, extending XF technology for studying T cell activation. The assay offers rapid, standardized measurement of early activation-associated metabolic responses in human T cells. It provides insights into activation dynamics and metabolic changes, supporting immune cell therapies and drug discovery. The assay includes two experimental designs: a standard assay for testing T cell activation and a modulation assay for compound effects on activation. Examples demonstrate its application in T cell immunology and metabolism.
Detect and modulate human T cell activation using the Agilent Seahorse XF Hu T Cell Activation Assay Kit
In this application note, Agilent technologies presents a novel approach to detect and modulate human T cell activation using the Seahorse XF Hu T Cell Activation Assay. This rapid assay measures proton efflux rates (PER) associated with glycolytic pathway-dependent energy production, offering real-time observation and early detection of T cell activation. The assay is supported by Seahorse Analytics software for data analysis, and the kit includes validated reagents and protocols for robust results.
