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Rapid, Reliable Measurement of Cellular Proliferation and Toxic Compound Effects on 3D Spheroid Cultures Grown from Multiple Cancer Cell Lines with ATPlite™ 3D and ATPlite 1step 3D

PerkinElmer
17 May 2017

Cellular viability and toxicity assays are fundamental tools in the drug discovery process – used to evaluate both the potency of compounds, as well as their toxicity profiles for early drug safety assessment. Cells cultured in 3D microtissues exhibit inherent differences in cellular adhesion and require substantially stronger lysis conditions than is sufficient for traditional monolayer or suspension cultures to access the cells deeper inside each spheroid. In order to address this need, we developed ATPlite 3D and ATPlite 1step 3D assay kits specifically for use with 3D cell cultures.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.CytotoxicityCytotoxicity assays measure the toxic effects of substances on cells, often used in drug testing and environmental studies. These tests are crucial in determining the safety of chemicals and pharmaceutical compounds. Explore cytotoxicity testing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Cell ProliferationCell proliferation refers to the process by which cells divide and multiply. This is a key concept in cancer research, tissue regeneration, and immunology studies. Explore cell proliferation tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Drug DiscoveryDrug discovery is the process of identifying potential new medications, involving stages such as target identification, compound screening, and preclinical development. It relies on cutting-edge technologies like high-throughput screening, artificial intelligence, and molecular modeling to accelerate the identification of drug candidates. Drug discovery plays a pivotal role in developing new therapies for diseases ranging from cancer to rare genetic disorders. Browse our peer-reviewed product directory to find the latest drug discovery technologies, compare options, check customer feedback, and get pricing directly from manufacturers.SpheroidsSpheroids are clusters of cells that have been grown in 3D culture to be used as <i>in vitro</i> model systems. These 3D microtissues can be used for toxicology testing, DMPK studies and many other applications involving cell analysis.Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.

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