PID controlled constant RQ fermentation of <i>Pichia pastoris</i> in the DASbox Mini Bioreactor System

27 May 2021

In this application note, Eppendorf demonstrates the feasibility of constant RQ based feeding for protein production-oriented yeast fermentation using the DASbox® Mini Bioreactor System.

DASbox® Mini Bioreactor

Eppendorf

Small working volumes save valuable material and medium in process development and screening procedures. The Eppendorf DASbox Mini Bioreactor is a fully instrumented mini bioreactor featuring industry standard design and advanced control functionalities.

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Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.BioreactorYeastBioprocessingBioprocessing is the use of biological systems for the conversion of raw materials to desired products. This includes the research, development and manufacturing stages of production and can refer to food processing, small-molecule pharmaceutical manufacture, production of recombinant protein therapeutics, or the generation of renewable energy.
PID controlled constant RQ fermentation of <i>Pichia pastoris</i> in the DASbox Mini Bioreactor System