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<i>In Vitro</i> Modeling of Cytokine-Drug Interactions Using Micropatterned Co-Cultures Of Primary Hepatocytes and Kupffer Macrophages

Hepregen Corporation
21 May 2014

Better predictive models for iDILI would enable the preclinical elimination of drug candidates with hepatotoxic liabilities. This poster describes a method in which HepatoPac co-cultures are supplemented with primary Kupffer macrophages for use in evaluating cytokine- drug interactions. Drug- induced stresses may interact with cytokine signaling to cause hepatotoxciity.

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Human HepatoPac™ Kits

Hepregen Corporation

Human HepatoPac™ kits are an ideal way to conduct all your DMPK, Predictive Toxicology & Mechanistic Toxicology applications. Begin dosing as early as 3 days after receiving the kit! Use our Human HepatoPac™ Triple Donor plates for the ultimate in convenience. HepatoPac™ co-cultures derived from 3 independent and highly characterized donors are provided on a single plate (8-wells from each donor).HepatoPac™ kits can be used for any assay that can be run using sandwich, monoculture plates or suspension cultures. Due to the extended in vitro functionality of HepatoPac™ co-cultures, Hepregen kits enable studies such as low turnover clearance or chronic toxicity studies that could not otherwise be performed.Human HepatoPac™ Kits Features: HT-ready format; 24- and 96-well plates Proprietary micropatterened co-culture Superior data for both slowly and rapidly metabolized drugs Culture longevity of up to 4 weeks Kits contain: Human HepatoPac™ Co-Cultures Maintenance Media Application-specific Media and additives Instructions for culture maintenance and application-specific protocols On-site customer training included

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ADME-ToxicologyADME-toxicology (ADME-Tox) studies are used in pharmacology and pharmacokinetics to assess the activity/toxicity of drugs <i>in vivo</i> or <i>in vitro</i>. Find bioassays for absorption, distribution, metabolism, and excretion of drug molecules including cytotoxicity, transporter/permeability, metabolism and activity assays as well as hepatocytes and cell lines for ADME. Find the best ADME-toxicology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.HepatotoxicityToxicityToxicity is defined as the degree to which a substance can harm humans or animals. It is essential to study the toxicity of pharmaceutical products during the development process so that optimum dosage can be established for the administration of the drug.

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