Interrogating PRMT5, MLL4, METTL3/14, and NSP14 with the AptaFluor SAH Assay
30 Oct 2023In this application note from BellBrook Labs, explore how the Aptafluor® SAH Assay provides a reliable and robust tool for detecting activity of four methyltransferases (MTs) using various acceptor substrates, including peptides, DNA and RNA. The AptaFluor SAH Methyltransferase Assay is a high-throughput enzymatic assay for SAM-dependent methyltransferases. The assay relies on a naturally occurring SAH-sensing RNA aptamer, or riboswitch, that binds SAH with nanomolar affinity and exquisite selectivity.
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AptaFluor™ SAH Methyltransferase Assay
BellBrook LabsThe AptaFluor® SAH Methyltransferase Assay Kit enables ultra-sensitive, direct detection of SAH—the universal product of methyltransferase reactions—using a riboswitch-based TR-FRET format. With no coupling enzymes required, it delivers high signal-to-background, Z’ ≥ 0.7, and is fully HTS-compatible for drug discovery and inhibitor screening.