Improved Reproducibility in Fluorescence Detection With Flow-Cell Temperature Control

Indole-3-acetic acid, commonly known as auxin, is a growth inhibitor that plays a role in plant morphosis. A synthetic form of auxin is widely used as an herbicide and a fruit growth promoter. It is expected to make a significant contribution in increasing research focused on increasing crop yields and biomass. Generally, the intensity of fluorescence in a compound is temperature dependent. The intensity of fluorescence in Indole-3-acetic acid decreases as temperature rises. In this application note, indole-3-acetic acid was measured using an HPLC system with an FP-4020 fluorescence detector with a temperature controlled cell. The effects of fluctuation in room temperature and the benefits of cell temperature control are reported.